Antibodies, Proteins, Kits and Reagents for Life Science

338 390 ₸ Product size

100 µl 338 390 ₸

Order now and get it on Tuesday March 09, 2021

Anti-IMP3 antibody [EPR12021] (ab177477)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12021] to IMP3
Suitable for: WB, ICC/IF, IP, Flow Cyt
Knockout validated
Reacts with: Human

Product name

Anti-IMP3 antibody [EPR12021]
See all IMP3 primary antibodies
Description

Rabbit monoclonal [EPR12021] to IMP3
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment within Human IMP3 aa 1-250. The exact sequence is proprietary.
Database link: O00425
Positive control
- HeLa, BxPC-3 and 293T lysates; BxPC-3 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR12021
Isotype

IgG
Research areas

Western blot - Anti-IMP3 antibody [EPR12021] (ab177477)

Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: IGF2BP3 (IMP3) knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: HEK293 whole cell lysate (20 µg)

Lanes 1 - 4: Merged signal (red and green). Green - ab177477 observed at 70 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab177477 was shown to specifically react with IGF2BP3 (IMP3) in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when IGF2BP3 (IMP3) knockout cells were examined. Wild-type and IGF2BP3 (IMP3) knockout samples were subjected to SDS-PAGE. Ab177477 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.
Immunocytochemistry/ Immunofluorescence - Anti-IMP3 antibody [EPR12021] (ab177477)

Immunofluorescent analysis of BxPC-3 cells labeling IMP3 with ab177477 at 1/50 (green) and DAPI staining (blue).
Flow Cytometry - Anti-IMP3 antibody [EPR12021] (ab177477)

Flow cytometry analysis of BxPC-3 (human pancreas adenocarcinoma) cells labeling with purified ab177477 at 1/300 dilution ( 10ug/ml) (Red). Cells were fixed with 80% methanol and permeabilised with 0.1% Tween-20. A Goat anti rabbit IgG (Alexa Fluor® 488)(ab150077) )(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as a isotype control.Cell without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.
Western blot - Anti-IMP3 antibody [EPR12021] (ab177477)

All lanes : Anti-IMP3 antibody [EPR12021] (ab177477) at 1/1000 dilution

Lane 1 : HeLa lysate
Lane 2 : BxPC-3 lysate
Lane 3 : 293T lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 63 kDa
Immunoprecipitation - Anti-IMP3 antibody [EPR12021] (ab177477)

Immunoprecipitation of IMP3 from 293T cell lysate using ab177477 at 1/10.
Western blot - Anti-IMP3 antibody [EPR12021] (ab177477)

All lanes : Anti-IMP3 antibody [EPR12021] (ab177477) at 1/20000 dilution

Lane 1 : MDA-MB-231 (human breast adenocarcinoma) whole cell lysate
Lane 2 : MDA-MB-435S (human melanoma) whole cell lysate
Lane 3 : MCF-7 (human breast adenocarcinoma) whole cell lysate
Lane 4 : Human Fetal Kidney

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/2000 dilution

Predicted band size: 63 kDa
Observed band size: 69 kDa
why is the actual band size different from the predicted?

Blocking/Diluting buffer 5% NFDM/TBST

The protein expression of IMP-3 in MCF-7 cells is negative or undetectable (PMID: 22266872).

Exposure time:
Lane 1: 10 seconds
Lane 2-4: 5 seconds
Anti-IMP3 antibody [EPR12021] (ab177477)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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