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Anti-eIF4A1 antibody [EPR14505(B)] - BSA and Azide free (ab250208)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14505(B)] to eIF4A1 - BSA and Azide free
  • Suitable for: ICC, Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-eIF4A1 antibody [EPR14505(B)] - BSA and Azide free
    See all eIF4A1 primary antibodies

  • Description

    Rabbit monoclonal [EPR14505(B)] to eIF4A1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250208 is the carrier-free version of ab180506. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250208 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-eIF4A1 antibody [EPR14505(B)] - BSA and Azide free (ab250208)
    Western blot - Anti-eIF4A1 antibody [EPR14505(B)] - BSA and Azide free (ab250208)
    All lanes : Anti-eIF4A1 antibody [EPR14505(B)] - N-terminal (ab180506) at 1/2000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 46 kDa
    Observed band size: 46 kDa



    This data was developed using ab180506, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-eIF4A1 antibody [EPR14505(B)] - BSA and Azide free (ab250208)
    Western blot - Anti-eIF4A1 antibody [EPR14505(B)] - BSA and Azide free (ab250208)
    All lanes : Anti-eIF4A1 antibody [EPR14505(B)] - N-terminal (ab180506) at 1/10000 dilution

    Lane 1 : MCF7 cell lysate
    Lane 2 : K562 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 46 kDa
    Observed band size: 46 kDa



    This data was developed using ab180506, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-eIF4A1 antibody [EPR14505(B)] - BSA and Azide free (ab250208)
    Immunocytochemistry - Anti-eIF4A1 antibody [EPR14505(B)] - BSA and Azide free (ab250208)
    This data was developed using ab180506, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of MCF-7 cells labeling eIF4A1 (green) with ab180506 at 1/250 dilution. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor®488-conjugated goat anti rabbit IgG was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).
  • Flow Cytometry - Anti-eIF4A1 antibody [EPR14505(B)] - BSA and Azide free (ab250208)
    Flow Cytometry - Anti-eIF4A1 antibody [EPR14505(B)] - BSA and Azide free (ab250208)
    This data was developed using ab180506, the same antibody clone in a different buffer formulation.Flow cytometry analysis of eIF4A1 expression in K562 cells using ab180506 at 1/50 dilution (red) and a rabbit IgG as negative control (green). A FITC-conjugated goat anti-rabbit IgG was used as the secondary antibody at 1/75 dilution.
  • Anti-eIF4A1 antibody [EPR14505(B)] - BSA and Azide free (ab250208)
    Anti-eIF4A1 antibody [EPR14505(B)] - BSA and Azide free (ab250208)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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