Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD45 antibody [EPR20033] - BSA and Azide free (ab229292)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20033] to CD45 - BSA and Azide free
  • Suitable for: IP, WB, IHC-P
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-CD45 antibody [EPR20033] - BSA and Azide free
    See all CD45 primary antibodies

  • Description

    Rabbit monoclonal [EPR20033] to CD45 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: J774A.1, WEHI-231 and Jurkat whole cell lysates; mouse thymus and spleen lysates. IP: J774A.1 whole cell lysate. IHC-P: Human tonsil tissue and Mouse spleen tissue.

  • General notes

    ab229292 is the carrier-free version of ab208022. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab229292 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [EPR20033] - BSA and Azide free (ab229292)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [EPR20033] - BSA and Azide free (ab229292)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse spleen tissue sections labeling CD45 with purified ab208022 at 1/5000 dilution (0.14 µg/ml). Heat mediated antigen retrieval was performed. ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208022).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [EPR20033] - BSA and Azide free (ab229292)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [EPR20033] - BSA and Azide free (ab229292)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling CD45 with purified ab208022 at 1/5000 dilution (0.14 µg/ml). Heat mediated antigen retrieval was performed. ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208022).

  • Immunoprecipitation - Anti-CD45 antibody [EPR20033] - BSA and Azide free (ab229292)
    Immunoprecipitation - Anti-CD45 antibody [EPR20033] - BSA and Azide free (ab229292)

    CD45 was immunoprecipitated from 0.35 mg of J774A.1 (Mouse reticulum cell sarcoma macrophage cell line) whole cell lysate with ab208022 at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using ab208022 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: J774A.1 whole cell lysate 10µg (Input).

    Lane 2: ab208022 IP in J774A.1 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab208022 in J774A.1 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208022).

  • Western blot - Anti-CD45 antibody [EPR20033] - BSA and Azide free (ab229292)
    Western blot - Anti-CD45 antibody [EPR20033] - BSA and Azide free (ab229292)
    All lanes : Anti-CD45 antibody [EPR20033] (ab208022) at 1/1000 dilution

    Lane 1 : Mouse thymus lysate
    Lane 2 : Mouse spleen tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 145 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 3 minutes; Lane 2: 15 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208022).

  • Anti-CD45 antibody [EPR20033] - BSA and Azide free (ab229292)
    Anti-CD45 antibody [EPR20033] - BSA and Azide free (ab229292)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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