Anti-IMPDH1 antibody (ab33039)
Key features and details
- Rabbit polyclonal to IMPDH1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-IMPDH1 antibody
See all IMPDH1 primary antibodies -
Description
Rabbit polyclonal to IMPDH1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Xenopus laevis, Zebrafish
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Immunogen
Synthetic peptide corresponding to Human IMPDH1 aa 500 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab33038) -
General notes
The immunogen used for this product shares 86% homology with IMPDH2. Cross-reactivity with this protein has not been confirmed experimentally.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab33039 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Application notesICC/IF: 1/100 (please refer to abreview for further details).
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.Target
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Function
Rate limiting enzyme in the de novo synthesis of guanine nucleotides and therefore is involved in the regulation of cell growth. It may also have a role in the development of malignancy and the growth progression of some tumors. -
Tissue specificity
IMP type I is the main species in normal leukocytes and type II predominates over type I in the tumor. -
Pathway
Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1. -
Involvement in disease
Defects in IMPDH1 are the cause of retinitis pigmentosa type 10 (RP10) [MIM:180105]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP10 inheritance is autosomal dominant. -
Sequence similarities
Belongs to the IMPDH/GMPR family.
Contains 2 CBS domains. - Information by UniProt
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Database links
- Entrez Gene: 3614 Human
- Entrez Gene: 23917 Mouse
- Entrez Gene: 362329 Rat
- Omim: 146690 Human
- SwissProt: P20839 Human
- SwissProt: P50096 Mouse
- SwissProt: D3ZLZ7 Rat
- Unigene: 654401 Human
see all -
Alternative names
- IMDH1_HUMAN antibody
- IMP (inosine monophosphate) dehydrogenase 1 antibody
- IMP dehydrogenase 1 antibody
see all
Images
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Western blot - Anti-IMPDH1 antibody (ab33039)All lanes : Anti-IMPDH1 antibody (ab33039) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :Jurkat whole cell lysate (ab7899)
Lane 3 :A-431 whole cell lysate (ab7909)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat anti-rabbit IgG (H&L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 55 kDa -
Western blot - Anti-IMPDH1 antibody (ab33039)All lanes : Anti-IMPDH1 antibody (ab33039) at 1 µg/ml
Lane 1 :NIH/3T3 whole cell lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Ovary (Mouse) Tissue Lysate - normal tissue
Lane 4 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 55 kDa
Datasheets and documents
References (2)
ab33039 has been referenced in 2 publications.
- Dang W et al. Inhibition of Calcineurin or IMP Dehydrogenase Exerts Moderate to Potent Antiviral Activity against Norovirus Replication. Antimicrob Agents Chemother 61:N/A (2017). PubMed: 28807916
- Kim HR et al. A p53-inducible microRNA-34a downregulates Ras signaling by targeting IMPDH. Biochem Biophys Res Commun 418:682-8 (2012). WB ; Human . PubMed: 22301190
Images
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Western blot - Anti-IMPDH1 antibody (ab33039)All lanes : Anti-IMPDH1 antibody (ab33039) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :Jurkat whole cell lysate (ab7899)
Lane 3 :A-431 whole cell lysate (ab7909)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat anti-rabbit IgG (H&L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 55 kDa -
Western blot - Anti-IMPDH1 antibody (ab33039)All lanes : Anti-IMPDH1 antibody (ab33039) at 1 µg/ml
Lane 1 :NIH/3T3 whole cell lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Ovary (Mouse) Tissue Lysate - normal tissue
Lane 4 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 55 kDa
