Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 02, 2021

Anti-DDX39 antibody [EPR13508] (ab180857)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR13508] to DDX39
Suitable for: Flow Cyt, IHC-P, WB
Reacts with: Human

Product name

Anti-DDX39 antibody [EPR13508]
See all DDX39 primary antibodies
Description

Rabbit monoclonal [EPR13508] to DDX39
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human DDX39 aa 350 to the C-terminus (C terminal) (Cysteine residue). The exact sequence is proprietary.
Database link: O00148
Positive control
- Jurkat, A431, HeLa, HepG2, and Human seminoma lysates. Human breast and colon tissue. Permabilized Jurkat cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR13508
Isotype

IgG
Research areas

Western blot - Anti-DDX39 antibody [EPR13508] (ab180857)

All lanes : Anti-DDX39 antibody [EPR13508] (ab180857) at 1/5000 dilution

Lane 1 : Jurkat cell lysate
Lane 2 : A431 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : Human seminoma lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 49 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX39 antibody [EPR13508] (ab180857)

Immunohistochemical analysis of Human breast tissue, staining DDX39 with ab180857 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX39 antibody [EPR13508] (ab180857)

Immunohistochemical analysis of Human colon tissue, staining DDX39 with ab180857 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cytometry - Anti-DDX39 antibody [EPR13508] (ab180857)

Flow cytometry analysis of DDX39 expression in permeabilized Jurkat cells using ab180857 at 1/10 dilution (red) and a rabbit IgG as negative control (green).
Anti-DDX39 antibody [EPR13508] (ab180857)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com