Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸

Order now and get it on Friday March 12, 2021

Key features and details

Rabbit monoclonal [A2D4] to DUSP6
Suitable for: WB, ICC/IF, IHC-P
Reacts with: Mouse, Rat, Human
Isotype: IgG

Product name

Anti-DUSP6 antibody [A2D4]
See all DUSP6 primary antibodies
Description

Rabbit monoclonal [A2D4] to DUSP6
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Rat Human
IHC-P	Mouse Human
WB	Mouse

See all applications and species data

Immunogen

Synthetic peptide corresponding to Human DUSP6 aa 350 to the C-terminus.
Database link: Q16828

Run BLAST with Run BLAST with
Positive control
- WB: Mouse pancreas extract. IHC-P: Mouse brain and pancreas tissue; human gastric cancer and pancreas tissue. ICC/IF: PC-12, HeLa and NIH/3T3 cells.
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
Purity

Affinity purified
Clonality

Monoclonal
Clone number

A2D4
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUSP6 antibody [A2D4] (ab238512)

Paraffin-embedded mouse pancreas tissue stained for DUSP6 using ab238512 at 1/100 dilution in immunohistochemical analysis (40X lens).
Immunocytochemistry/ Immunofluorescence - Anti-DUSP6 antibody [A2D4] (ab238512)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for DUSP6 (green) using ab238512 at 1/50 dilution in ICC/IF.
Western blot - Anti-DUSP6 antibody [A2D4] (ab238512)

Anti-DUSP6 antibody [A2D4] (ab238512) at 1/500 dilution + Mouse pancreas extract at 25 µg

Secondary
HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 42 kDa

Blocking buffer: 3% non-fat dry milk in TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUSP6 antibody [A2D4] (ab238512)

Paraffin-embedded mouse brain tissue stained for DUSP6 using ab238512 at 1/100 dilution in immunohistochemical analysis (40X lens).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUSP6 antibody [A2D4] (ab238512)

Paraffin-embedded human gastric cancer tissue stained for DUSP6 using ab238512 at 1/100 dilution in immunohistochemical analysis (40X lens).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUSP6 antibody [A2D4] (ab238512)

Paraffin-embedded human pancreas tissue stained for DUSP6 using ab238512 at 1/100 dilution in immunohistochemical analysis (40X lens).
Immunocytochemistry/ Immunofluorescence - Anti-DUSP6 antibody [A2D4] (ab238512)

PC-12 (rat adrenal gland pheochromocytoma cell line) cells stained for DUSP6 (green) using ab238512 at 1/50 dilution in ICC/IF.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUSP6 antibody [A2D4] (ab238512)

NIH/3T3 (mouse embryo fibroblast cell line) cells stained for DUSP6 (green) using ab238512 at 1/50 dilution in ICC/IF.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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