Anti-14-3-3 alpha + beta antibody - C-terminal (ab216618)
Key features and details
- Rabbit polyclonal to 14-3-3 alpha + beta - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-14-3-3 alpha + beta antibody - C-terminal
See all 14-3-3 alpha + beta primary antibodies -
Description
Rabbit polyclonal to 14-3-3 alpha + beta - C-terminal -
Host species
Rabbit -
Specificity
This product cross-reacts with 14-3-3 gamma, delta and epsilon. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Mouse
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Immunogen
Synthetic peptide aa 200 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P31946 -
Positive control
- Mouse brain lysate; Human rectal carcinoma tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-14-3-3 alpha + beta antibody - C-terminal (ab216618)All lanes : Anti-14-3-3 alpha + beta antibody - C-terminal (ab216618) at 1/200 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse kidney lysate
Secondary
All lanes : Goat anti-rabbit IgG (H+L), HRP conjugated at 1/3000 dilution
Predicted band size: 28 kDa
Observed band size: 31 kDa why is the actual band size different from the predicted?12% gel
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 alpha + beta antibody - C-terminal (ab216618)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human rectal carcinoma tissue labeling 14-3-3 alpha + beta with ab216618 at 1/200 dilution, followed by Goat anti-Rabbit IgG, PE conjugted at 1/200 dilution.
