Anti-14-3-3 alpha + beta antibody (ab237807)
Key features and details
- Rabbit polyclonal to 14-3-3 alpha + beta
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human, Zebrafish
- Isotype: IgG
Overview
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Product name
Anti-14-3-3 alpha + beta antibody
See all 14-3-3 alpha + beta primary antibodies -
Description
Rabbit polyclonal to 14-3-3 alpha + beta -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Zebrafish
Predicted to work with: Cow, Cynomolgus monkey, Orangutan
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Immunogen
Recombinant full length protein corresponding to Human 14-3-3 alpha + beta aa 1 to the C-terminus.
Database link: P31946 -
Positive control
- WB: HEK-293T, EC109, HeLa, Raji, HepG2, Jurkat and HT-29 whole cell lysates; Rat brain, liver and lung lysates; mouse liver, brain and lung lysates; Zebrafish lysate. IHC-P: Human kidney, testis and small intestine tissues. ICC/IF: HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity greater than 95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-14-3-3 alpha + beta antibody (ab237807)All lanes : Anti-14-3-3 alpha + beta antibody (ab237807) at 1/500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : EC109 (Human esophageal squamous cell carcinoma cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to Rabbit IgG at 1/15000 dilution
Predicted band size: 28 kDa
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Western blot - Anti-14-3-3 alpha + beta antibody (ab237807)All lanes : Anti-14-3-3 alpha + beta antibody (ab237807) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 3 : Mouse liver lysate
Lane 4 : Mouse brain lysate
Lane 5 : Rat liver lysate
Lane 6 : Rat lung lysate
Lane 7 : Mouse lung lysate
Lane 8 : Zebrafish lysate
Secondary
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 28 kDa
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Western blot - Anti-14-3-3 alpha + beta antibody (ab237807)All lanes : Anti-14-3-3 alpha + beta antibody (ab237807) at 1/500 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 28 kDa
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Western blot - Anti-14-3-3 alpha + beta antibody (ab237807)All lanes : Anti-14-3-3 alpha + beta antibody (ab237807) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 5 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 6 : HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate
Lane 7 : Rat brain lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 28 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 alpha + beta antibody (ab237807)Paraffin-embedded human kidney tissue stained for 14-3-3 alpha + beta using ab237807 at 1/600 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 alpha + beta antibody (ab237807)Paraffin-embedded human testis tissue stained for 14-3-3 alpha + beta using ab237807 at 1/600 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 alpha + beta antibody (ab237807)Paraffin-embedded human small intestine tissue stained for 14-3-3 alpha + beta using ab237807 at 1/400 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
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Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 alpha + beta antibody (ab237807)HepG2 (human liver hepatocellular carcinoma cell line) cells stained for 14-3-3 alpha + beta (green) using ab237807 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
