Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Exportin-5 antibody [EPR8452] (ab129006)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8452] to Exportin-5
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Exportin-5 antibody [EPR8452]
    See all Exportin-5 primary antibodies

  • Description

    Rabbit monoclonal [EPR8452] to Exportin-5

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Exportin-5 aa 100-200. The exact sequence is proprietary.

  • Positive control

    • HeLa, K562, C6, RAW264.7, PC12, and NIH 3T3 cell lysates; Human fetal heart and brain lysates; Human colon tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Exportin-5 antibody [EPR8452] (ab129006)
    Western blot - Anti-Exportin-5 antibody [EPR8452] (ab129006)
    All lanes : Anti-Exportin-5 antibody [EPR8452] (ab129006) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : Human fetal heart lysate
    Lane 4 : Human fetal brain lysate
    Lane 5 : C6 cell lysate
    Lane 6 : RAW264.7 cell lysate
    Lane 7 : PC12 cell lysate
    Lane 8 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 136 kDa
    Observed band size: 136 kDa

  • Flow Cytometry - Anti-Exportin-5 antibody [EPR8452] (ab129006)
    Flow Cytometry - Anti-Exportin-5 antibody [EPR8452] (ab129006)
    Overlay histogram showing HEK293 cells stained with ab129006 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab129006, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Exportin-5 antibody [EPR8452] (ab129006)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Exportin-5 antibody [EPR8452] (ab129006)

    ab129006, at 1/250 dilution, staining Exportin-5 in paraffin-embedded Human colon tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-Exportin-5 antibody [EPR8452] (ab129006)
    OI-RD Scanning - Anti-Exportin-5 antibody [EPR8452] (ab129006)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Exportin-5 antibody [EPR8452] (ab129006)
    Anti-Exportin-5 antibody [EPR8452] (ab129006)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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