Anti-Carbonic Anhydrase 3/CA3 antibody [EPR13425] (ab181358)
![Anti-Carbonic Anhydrase 3/CA3 antibody [EPR13425] (ab181358)](https://www.abcam.com/ps/products/181/ab181358/Images/ab181358-210237-anti-carbonic-anhydrase-iii-antibody-epr13425-immunohistochemistry.jpg "Anti-Carbonic Anhydrase 3/CA3 antibody [EPR13425] (ab181358)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13425] to Carbonic Anhydrase 3/CA3
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-Carbonic Anhydrase 3/CA3 antibody [EPR13425]
See all Carbonic Anhydrase 3/CA3 primary antibodies -
Description
Rabbit monoclonal [EPR13425] to Carbonic Anhydrase 3/CA3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
-
Immunogen
Synthetic peptide within Human Carbonic Anhydrase 3/CA3 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
Database link: P07451 -
Positive control
- Human skeletal muscle, rat muscle, and mouse muscle lysates, Human skeletal muscle tissue
-
General notes
This product was previously labelled as Carbonic Anhydrase III
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13425 -
Isotype
IgG -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 3/CA3 antibody [EPR13425] (ab181358)
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Carbonic Anhydrase 3/CA3 with ab181358 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Western blot - Anti-Carbonic Anhydrase 3/CA3 antibody [EPR13425] (ab181358)](https://www.abcam.com/ps/products/181/ab181358/Images/ab181358-210220-anti-carbonic-anhydrase-iii-antibody-epr13425-western-blot.jpg)
Western blot - Anti-Carbonic Anhydrase 3/CA3 antibody [EPR13425] (ab181358)All lanes : Anti-Carbonic Anhydrase 3/CA3 antibody [EPR13425] (ab181358) at 1/1000 dilution
Lane 1 : Human skeletal muscle lysates
Lane 2 : Rat muscle lysates
Lane 3 : Mouse muscle lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 29 kDa
-
![Anti-Carbonic Anhydrase 3/CA3 antibody [EPR13425] (ab181358)](https://www.abcam.com/ps/products/181/ab181358/Images/ab181358-3-benefits-of-recombinant-antibodies.png)
Anti-Carbonic Anhydrase 3/CA3 antibody [EPR13425] (ab181358)
