Anti-Carbonic Anhydrase 3/CA3 antibody (ab150037)
Key features and details
- Rabbit polyclonal to Carbonic Anhydrase 3/CA3
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-Carbonic Anhydrase 3/CA3 antibody
See all Carbonic Anhydrase 3/CA3 primary antibodies -
Description
Rabbit polyclonal to Carbonic Anhydrase 3/CA3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Recombinant fragment
Predicted to work with: Chimpanzee, Macaque monkey, Chinese hamster, Orangutan
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Immunogen
Synthetic peptide corresponding to Human Carbonic Anhydrase 3/CA3 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab136233) -
Positive control
- This antibody gave a positive signal in Recombinant Carbonic anhydrase 3 protein as well as the following tissue lysates: Human Skeletal Muscle; Mouse Skeletal Muscle; Rat Skeletal Muscle. It also gave a positive result in FFPE human skeletal muscle tissue sections.
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General notes
This product was previously labelled as Carbonic Anhydrase III
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab150037 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 29 kDa). IHC-P Use a concentration of 0.5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Target
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Function
Reversible hydration of carbon dioxide. -
Tissue specificity
Muscle specific. -
Sequence similarities
Belongs to the alpha-carbonic anhydrase family. -
Developmental stage
At 6 weeks gestation, transcripts accumulate at low levels in the somites and at high levels throughout the notochord. As gestation continues, CA3 becomes abundant in all developing muscle masses and continues at high to moderate levels in the notochord. -
Post-translational
modificationsS-glutathionylated in hepatocytes under oxidative stress. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 761 Human
- Entrez Gene: 12350 Mouse
- Entrez Gene: 54232 Rat
- Omim: 114750 Human
- SwissProt: P07451 Human
- SwissProt: P16015 Mouse
- SwissProt: P14141 Rat
- Unigene: 82129 Human
see all -
Alternative names
- CA III antibody
- CA-III antibody
- Ca3 antibody
see all
Images
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Western blot - Anti-Carbonic Anhydrase 3/CA3 antibody (ab150037)All lanes : Anti-Carbonic Anhydrase 3/CA3 antibody (ab150037) at 1 µg/ml
Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate
Lane 3 : Skeletal Muscle (Rat) Tissue Lysate
Lane 4 : Carbonic Anhydrase III/CA3 protein (ab50779)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
Additional bands at: 24 kDa, 58 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 3/CA3 antibody (ab150037)IHC image of Carbonic Anydrase III staining in human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab150037, 0.5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
References (0)
ab150037 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-Carbonic Anhydrase 3/CA3 antibody (ab150037)All lanes : Anti-Carbonic Anhydrase 3/CA3 antibody (ab150037) at 1 µg/ml
Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate
Lane 3 : Skeletal Muscle (Rat) Tissue Lysate
Lane 4 : Carbonic Anhydrase III/CA3 protein (ab50779)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
Additional bands at: 24 kDa, 58 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 3/CA3 antibody (ab150037)IHC image of Carbonic Anydrase III staining in human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab150037, 0.5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
