Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MUM1 antibody [EPR5653] (ab124691)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5653] to MUM1
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-MUM1 antibody [EPR5653]
    See all MUM1 primary antibodies

  • Description

    Rabbit monoclonal [EPR5653] to MUM1

  • Host species

    Rabbit

  • Specificity

    Uniprot ID - Q15306

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human MUM1 (N terminal). The exact sequence is proprietary.

  • Positive control

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUM1 antibody [EPR5653] (ab124691)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUM1 antibody [EPR5653] (ab124691)

    ab124691, at 1/100, staining MUM1 in paraffin-embedded Human tonsil tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-MUM1 antibody [EPR5653] (ab124691)
    Western blot - Anti-MUM1 antibody [EPR5653] (ab124691)
    All lanes : Anti-MUM1 antibody [EPR5653] (ab124691) at 1/1000 dilution

    Lane 1 : Ramos cell lysate
    Lane 2 : HuT 78 cell lysate
    Lane 3 : IM 9 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 52 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUM1 antibody [EPR5653] (ab124691)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUM1 antibody [EPR5653] (ab124691)

    ab124691, at 1/100, staining MUM1 in paraffin-embedded Human colon tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUM1 antibody [EPR5653] (ab124691)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUM1 antibody [EPR5653] (ab124691) This image is courtesy of an anonymous Abreview
    Immunohistochemical analysis of dog spleen tissue, staining MUM1 with ab124691.

    Tissue was fixed with formaldehyde and blocked with 2.5% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation in a TRIS-EDTA buffer (pH 9). Samples were incubated with primary antibody (1/50 in PBS plus 1X casein) for 1 hour 30 minutes at 37°C. An undiluted HRP-conjugated horse anti-rabbit polyclonal IgG was used as the secondary antibody.

    See Abreview

  • OI-RD Scanning - Anti-MUM1 antibody [EPR5653] (ab124691)
    OI-RD Scanning - Anti-MUM1 antibody [EPR5653] (ab124691)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-MUM1 antibody [EPR5653] (ab124691)
    Anti-MUM1 antibody [EPR5653] (ab124691)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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