Antibodies, Proteins, Kits and Reagents for Life Science

281 433 ₸ Product size

100 µl 281 433 ₸ 1 ml 465 705 ₸

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Anti-MUM1 antibody [SP114] - C-terminal (ab227646)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [SP114] to MUM1 - C-terminal
Suitable for: WB, IHC-P, Flow Cyt
Reacts with: Human

Product name

Anti-MUM1 antibody [SP114] - C-terminal
See all MUM1 primary antibodies
Description

Rabbit monoclonal [SP114] to MUM1 - C-terminal
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human MUM1 aa 100 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q15306
Positive control
- IHC-P: Hodgkins lymphoma, HK lymphoma, " Human Hodgkins lymphoma", and Human colon tissue; WB: Ramos, 293T, IM-9 and HuT-78 cell lysate Flow Cyt: Ramos cells.
General notes

This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
Purity

Protein A/G purified
Purification notes

Purified from TCS by protein A/G.
Clonality

Monoclonal
Clone number

SP114
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUM1 antibody [SP114] - C-terminal (ab227646)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling MUM1 with ab227646 at 1/100 dilution (2.32 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Western blot - Anti-MUM1 antibody [SP114] - C-terminal (ab227646)

All lanes : Anti-MUM1 antibody [SP114] - C-terminal (ab227646) at 0.25 µg/ml

Lane 1 : Ramos (Human Burkitt's lymphoma B lymphocyte) cell lysate
Lane 2 : HuT-78 (Human Sezary syndrome cutaneous T lymphocyte) cell lysate
Lane 3 : IM-9 (Human peripheral blood lymphoblast) cell lysate
Lane 4 : 293T (Human embryonic kidney epithelial cell) cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml

Predicted band size: 52 kDa
Observed band size: 52 kDa

Blocking/Diluting buffer and concentration: 5% NFDM /TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUM1 antibody [SP114] - C-terminal (ab227646)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human Hodgkin's lymphoma tissue sections labeling MUM1 with ab227646 at 1/100 dilution (2.32 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUM1 antibody [SP114] - C-terminal (ab227646)

Formalin-fixed, paraffin-embedded human Hodgkin's lymphoma tissue stained for MUM1 using ab227646 at 1/100 dilution in immunohistochemical analysis.
Flow Cytometry - Anti-MUM1 antibody [SP114] - C-terminal (ab227646)

Flow Cytometry analysis of Ramos(Human Burkitt's lymphoma B lymphocyte) cells labeling MUM1 with purified ab227646 at 1:230 dilution (1.01 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.
Western blot - Anti-MUM1 antibody [SP114] - C-terminal (ab227646)

Anti-MUM1 antibody [SP114] - C-terminal (ab227646) at 1/100 dilution + Ramos (human Burkitt's lymphoma cell line) cell lysate

Predicted band size: 52 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUM1 antibody [SP114] - C-terminal (ab227646)

Formalin-fixed, paraffin-embedded human HK lymphoma tissue stained for MUM1 using ab227646 at 1/100 dilution in immunohistochemical analysis.
Anti-MUM1 antibody [SP114] - C-terminal (ab227646)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com