Anti-TRAF3 antibody [EPR22992-93] (ab239357)
![Anti-TRAF3 antibody [EPR22992-93] (ab239357)](https://www.abcam.com/ps/products/239/ab239357/Images/ab239357-355081-anti-traf3-antibody-epr22992-93-western-blot-2.jpg "Anti-TRAF3 antibody [EPR22992-93] (ab239357)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22992-93] to TRAF3
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-TRAF3 antibody [EPR22992-93]
See all TRAF3 primary antibodies -
Description
Rabbit monoclonal [EPR22992-93] to TRAF3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Wild type HAP1 whole cell lysate. Daudi, HEK-293T, C6, RAW264.7, PC-12 and NIH/3T3 whole cell lysate. Mouse brain, rat spleen and rat brain lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22992-93 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TRAF3 antibody [EPR22992-93] (ab239357)All lanes : Anti-TRAF3 antibody [EPR22992-93] (ab239357) at 1/1000 dilution
Lane 1 : Wild type HAP1 whole cell lysate
Lane 2 : TRAF3 knockout HAP1 whole cell lysate
Lane 3 : Daudi (human Burkitts lymphoma lymphoblast), whole cell lysate
Lane 4 : HEK-293T (human embryonic kidney epithelial cell), whole cell lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 64 kDa
Observed band size: 64 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
ab239357 was shown to specifically react with TRAF3 in wild-type HAP1 cells as signal was lost in TRAF3 knockout cells. Wild-type and TRAF3 knockout samples were subjected to SDS-PAGE. ab239357 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/20,000 dilution for 1 hour at room temperature before imaging. Exposure time: 37 seconds.
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![Western blot - Anti-TRAF3 antibody [EPR22992-93] (ab239357)](https://www.abcam.com/ps/products/239/ab239357/Images/ab239357-355083-anti-traf3-antibody-epr22992-93-western-blot.jpg)
Western blot - Anti-TRAF3 antibody [EPR22992-93] (ab239357)All lanes : Anti-TRAF3 antibody [EPR22992-93] (ab239357) at 1/1000 dilution
Lane 1 : C6 (rat glial tumor glial cell), whole cell lysate
Lane 2 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
Lane 3 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate
Lane 4 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lane 5 : Mouse brain tissue lysate
Lane 6 : Rat spleen tissue lysate
Lane 7 : Rat brain tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 64 kDa
Observed band size: 64 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: Lanes 1-4: 128 seconds; Lane 5: 3 minutes; Lane 6: 125 seconds; Lane 7: 8 seconds.
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![Anti-TRAF3 antibody [EPR22992-93] (ab239357)](https://www.abcam.com/ps/products/239/ab239357/Images/ab239357-3-benefits-of-recombinant-antibodies.png)
Anti-TRAF3 antibody [EPR22992-93] (ab239357)
