Anti-TRAF3 antibody [EPR22992-93] - BSA and Azide free (ab263026)
![Anti-TRAF3 antibody [EPR22992-93] - BSA and Azide free (ab263026)](https://www.abcam.com/ps/products/263/ab263026/Images/ab263026-355105-AntiTRAF3-antibody-EPR2299293--BSA-and-Azide-free-western-blot-knock-out.jpg "Anti-TRAF3 antibody [EPR22992-93] - BSA and Azide free (ab263026)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22992-93] to TRAF3 - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-TRAF3 antibody [EPR22992-93] - BSA and Azide free
See all TRAF3 primary antibodies -
Description
Rabbit monoclonal [EPR22992-93] to TRAF3 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Wild type HAP1 whole cell lysate. Daudi, HEK-293T, C6, RAW264.7, PC-12 and NIH/3T3 whole cell lysate. Mouse brain, rat spleen and rat brain lysates.
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General notes
ab263026 is the carrier-free version of ab239357 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab263026 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22992-93 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TRAF3 antibody [EPR22992-93] - BSA and Azide free (ab263026)All lanes : Anti-TRAF3 antibody [EPR22992-93] (ab239357) at 1/1000 dilution
Lane 1 : Wild type HAP1 whole cell lysate
Lane 2 : TRAF3 knockout HAP1 whole cell lysate
Lane 3 : Daudi (human Burkitt's lymphoma lymphoblast), whole cell lysate
Lane 4 : HEK-293T (human embryonic kidney epithelial cell), whole cell lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 64 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
ab239357 was shown to specifically react with TRAF3 in wild-type HAP1 cells as signal was lost in TRAF3 knockout cells. Wild-type and TRAF3 knockout samples were subjected to SDS-PAGE. ab239357 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/20,000 dilution for 1 hour at room temperature before imaging. Exposure time: 37 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab239357).
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![Anti-TRAF3 antibody [EPR22992-93] - BSA and Azide free (ab263026)](https://www.abcam.com/ps/products/263/ab263026/Images/ab263026-2-benefits-of-recombinant-antibodies.png)
Anti-TRAF3 antibody [EPR22992-93] - BSA and Azide free (ab263026)
