Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RKIP antibody [EPR2875Y] (ab76582)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2875Y] to RKIP
  • Suitable for: Flow Cyt, IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-RKIP antibody [EPR2875Y]
    See all RKIP primary antibodies

  • Description

    Rabbit monoclonal [EPR2875Y] to RKIP

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Mouse
    Rat
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human RKIP aa 50-150. The exact sequence is proprietary.

  • Positive control

    • WB: HeLa, A549, HepG2, A431, mouse brain, mouse testis and rat brain cell lysates; IHC-P: human ovarian carcinoma and brain, mouse kidney, and rat testis tissues; ICC/IF: HepG2 cells; Flow Cyt: HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-RKIP antibody [EPR2875Y] (ab76582)
    Western blot - Anti-RKIP antibody [EPR2875Y] (ab76582)
    All lanes : Anti-RKIP antibody [EPR2875Y] (ab76582) at 1/1000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : A549 (Human lung carcinoma epithelial cell) whole cell lysates
    Lane 3 : Mouse brain lysates
    Lane 4 : Mouse testis lysates
    Lane 5 : Rat brain lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 21 kDa
    Observed band size: 21 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-RKIP antibody [EPR2875Y] (ab76582)
    Immunocytochemistry/ Immunofluorescence - Anti-RKIP antibody [EPR2875Y] (ab76582)
    Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling RKIP with purified ab76582 at 1:50 dilution (2.6 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RKIP antibody [EPR2875Y] (ab76582)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RKIP antibody [EPR2875Y] (ab76582)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat testis tissue sections labeling RKIP with purified ab76582 at 1/500 dilution (0.26 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Flow Cytometry - Anti-RKIP antibody [EPR2875Y] (ab76582)
    Flow Cytometry - Anti-RKIP antibody [EPR2875Y] (ab76582)

    Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling RKIP (red) with purified ab76582 at a 1/20 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RKIP antibody [EPR2875Y] (ab76582)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RKIP antibody [EPR2875Y] (ab76582)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling RKIP with purified ab76582 at 1/500 dilution (0.26 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RKIP antibody [EPR2875Y] (ab76582)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RKIP antibody [EPR2875Y] (ab76582)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue sections labeling RKIP with purified ab76582 at 1/500 dilution (0.26 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Western blot - Anti-RKIP antibody [EPR2875Y] (ab76582)
    Western blot - Anti-RKIP antibody [EPR2875Y] (ab76582)
    All lanes : Anti-RKIP antibody [EPR2875Y] (ab76582) at 1/5000 dilution (unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : A549 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : A431 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 21 kDa
    Observed band size: 21 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RKIP antibody [EPR2875Y] (ab76582)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RKIP antibody [EPR2875Y] (ab76582)

    Staining of formalin-fixed, paraffin-embedded human brain tissue using 1/250 ab76582 (unpurified).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-RKIP antibody [EPR2875Y] (ab76582)
    Western blot - Anti-RKIP antibody [EPR2875Y] (ab76582)
    Anti-RKIP antibody [EPR2875Y] (ab76582) at 1/5000 dilution (unpurified) + Recombinant Human RKIP protein (ab87892) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 21 kDa


    Exposure time: 30 seconds
  • Anti-RKIP antibody [EPR2875Y] (ab76582)
    Anti-RKIP antibody [EPR2875Y] (ab76582)

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