Anti-RKIP antibody (ab109101)
Key features and details
- Rabbit polyclonal to RKIP
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RKIP antibody
See all RKIP primary antibodies -
Description
Rabbit polyclonal to RKIP -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive signal in Mouse Hippocampus, Human Brain and Human Spinal Cord tissue lysates, as well as the following whole cell lysates: HeLa; A549; HepG2; A431; HL60.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109101 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseAll applications RabbitHorseChickenCowPigChimpanzeeMacaque monkeyGorillaOrangutanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).Target
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Function
Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase.
HCNP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase. Seems to be mediated by a specific receptor. -
Sequence similarities
Belongs to the phosphatidylethanolamine-binding protein family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 431786 Cow
- Entrez Gene: 5037 Human
- Entrez Gene: 23980 Mouse
- Entrez Gene: 100173932 Orangutan
- Entrez Gene: 100008884 Rabbit
- Omim: 604591 Human
- SwissProt: P13696 Cow
- SwissProt: P30086 Human
see all -
Alternative names
- Epididymis luminal protein 210 antibody
- Epididymis secretory protein Li 34 antibody
- Epididymis secretory protein Li 96 antibody
see all
Images
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Western blot - Anti-RKIP antibody (ab109101)All lanes : Anti-RKIP antibody (ab109101) at 1 µg/ml
Lane 1 : Mouse Hippocampus Tissue Lysate
Lane 2 : Human brain tissue lysate - total protein (ab29466)
Lane 3 : Human spinal cord tissue lysate - total protein (ab29188)
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 5 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 7 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 8 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 21 kDa
Observed band size: 21 kDa
Additional bands at: 18 kDa, 34 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
Datasheets and documents
References (0)
ab109101 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-RKIP antibody (ab109101)All lanes : Anti-RKIP antibody (ab109101) at 1 µg/ml
Lane 1 : Mouse Hippocampus Tissue Lysate
Lane 2 : Human brain tissue lysate - total protein (ab29466)
Lane 3 : Human spinal cord tissue lysate - total protein (ab29188)
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 5 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 7 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 8 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 21 kDa
Observed band size: 21 kDa
Additional bands at: 18 kDa, 34 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
