Anti-Cytochrome P450 2E1 antibody (ab53945)
Key features and details
- Rabbit polyclonal to Cytochrome P450 2E1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cytochrome P450 2E1 antibody
See all Cytochrome P450 2E1 primary antibodies -
Description
Rabbit polyclonal to Cytochrome P450 2E1 -
Host species
Rabbit -
Specificity
ab53945 recognizes Cytochrome P450 2E1. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
Synthetic peptide (Human) Cytochrome P450 2E1
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Positive control
- IHC-P: Human liver carcinoma WB: Pyridine-treated kidney microsome.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Cytochrome P450 2E1 antibody (ab53945)All lanes : Anti-Cytochrome P450 2E1 antibody (ab53945) at 1/1000 dilution
Lane 1 : Control kidney microsome
Lane 2 : Pyridine-treated kidney microsome
Lysates/proteins at 10 µg per lane.
Predicted band size: 57 kDa
Observed band size: 57 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 2E1 antibody (ab53945)IHC image of ab53945 staining in human liver carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab53945, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
