Anti-Cytochrome P450 2E1 antibody (ab28146)
Key features and details
- Rabbit polyclonal to Cytochrome P450 2E1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Rabbit, Human
- Isotype: IgG
Overview
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Product name
Anti-Cytochrome P450 2E1 antibody
See all Cytochrome P450 2E1 primary antibodies -
Description
Rabbit polyclonal to Cytochrome P450 2E1 -
Host species
Rabbit -
Specificity
The antibody detects a ~50-55 kDa protein, corresponding to the apparent molecular mass of cytochrome P450 IIE1 on SDS-PAGE immunoblots. -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Rabbit, Human -
Immunogen
Full length native protein (purified) (Rat)
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General notes
The antibody has been shown to inhibit microsomal benzene metabolism
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Primary antibody notes
The antibody has been shown to inhibit microsomal benzene metabolism -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Cytochrome P450 2E1 antibody (ab28146)All lanes : Anti-Cytochrome P450 2E1 antibody (ab28146) at 1/1000 dilution
Lane 1 : Molecular weight marker
Lane 2 : Cell lysates prepared from human liver microsomes
Lane 3 : Cell lysates prepared from rat liver microsomes
Lane 4 : Cell lysates prepared from mouse liver microsomes
Lane 5 : Cell lysates prepared from rabbit liver microsomes
Predicted band size: 50-55 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 2E1 antibody (ab28146)ICC/IF image of ab28146 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28146, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-Cytochrome P450 2E1 antibody (ab28146) Image courtesy of an anonymous Abreview.All lanes : Anti-Cytochrome P450 2E1 antibody (ab28146) at 1/2500 dilution
All lanes : Tissue lysate prepared from normal murine liver
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG-HRP at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 50-55 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 3 seconds
