Antibodies, Proteins, Kits and Reagents for Life Science

341 740 ₸ Product size

100 µl 341 740 ₸

Order now and get it on Tuesday March 09, 2021

Anti-HSPB7 antibody [EPR10106(B)] (ab150390)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10106(B)] to HSPB7
Suitable for: IHC-P, WB, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-HSPB7 antibody [EPR10106(B)]
See all HSPB7 primary antibodies
Description

Rabbit monoclonal [EPR10106(B)] to HSPB7
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Mouse
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide within Human HSPB7 aa 1-100. The exact sequence is proprietary.
Positive control
- Human fetal heart cell lysate; Human skeletal muscle and heart tissues, mouse and rat heart tissue lysates.
General notes
This product was previously labelled as cvHSP

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.14% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR10106(B)
Isotype

IgG
Research areas

Western blot - Anti-HSPB7 antibody [EPR10106(B)] (ab150390)

All lanes : Anti-HSPB7 antibody [EPR10106(B)] (ab150390) at 1/1000 dilution

Lane 1 : Mouse heart tissue lysates
Lane 2 : Rat heart tissue lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 19 kDa
Additional bands at: 19 kDa. We are unsure as to the identity of these extra bands.
Western blot - Anti-HSPB7 antibody [EPR10106(B)] (ab150390)

All lanes : Anti-HSPB7 antibody [EPR10106(B)] (ab150390) at 1/1000 dilution

Lane 1 : Human fetal heart tissue lysates
Lane 2 : Human skeletal muscle tissue lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 19 kDa
Additional bands at: 19 kDa. We are unsure as to the identity of these extra bands.
Western blot - Anti-HSPB7 antibody [EPR10106(B)] (ab150390) This image is courtesy of an anonymous Abreview

All lanes : Anti-HSPB7 antibody [EPR10106(B)] (ab150390) at 1/200 dilution

All lanes : Mouse embryonic heart lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit polyclonal HRP conjugated antibody at 1/3000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 19 kDa

Exposure time: 2 minutes

Bocking carried out at 4^oC for 12 hours with 2.5% BSA in TBST. Dilutions carried out in Blocking buffer.
Gel used 4-12%
See Abreview
Western blot - Anti-HSPB7 antibody [EPR10106(B)] (ab150390)

Anti-HSPB7 antibody [EPR10106(B)] (ab150390) at 1/1000 dilution + Human fetal heart cell lysate at 10 µg

Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 19 kDa
Immunoprecipitation - Anti-HSPB7 antibody [EPR10106(B)] (ab150390)

ab150390 immunoprecipitating HSPB7. 10µg of cell lysate was incubated with primary antibody at a dilution of 1/20 and VeriBlot for IP Detection Reagent (HRP) (ab131366) at a dilution of 1/10000.

Lane 1: Mouse heart whole cell lysate (10ug)
Lane 2: Mouse heart whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab150390 in mouse heart whole cell lysate
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPB7 antibody [EPR10106(B)] (ab150390)

Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue labeling HSPB7 with ab150390 at a 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPB7 antibody [EPR10106(B)] (ab150390)

ab150390 staining HSPB7 in human skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/50. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

Negative control 1: PBS in place of primary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPB7 antibody [EPR10106(B)] (ab150390)

Immunohistochemical analysis of paraffin embedded Human Fetal heart tissue using ab150390 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPB7 antibody [EPR10106(B)] (ab150390)

Immunohistochemical analysis of paraffin embedded Human heart tissue labeling HSPB7 with ab150390 at a 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPB7 antibody [EPR10106(B)] (ab150390)

ab150390 staining HSPB7 in human cerebral cortex tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/50. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

Negative control 1: PBS in place of primary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPB7 antibody [EPR10106(B)] (ab150390)

Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using ab150390 showing -ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPB7 antibody [EPR10106(B)] (ab150390)

Immunohistochemical analysis of paraffin embedded normal Human colon tissue using ab150390 showing -ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPB7 antibody [EPR10106(B)] (ab150390)

Immunohistochemical analysis of paraffin embedded normal Human kidney tissue using ab150390 showing -ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Anti-HSPB7 antibody [EPR10106(B)] (ab150390)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com