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Anti-MUM1 antibody [SP114] - BSA and Azide free (ab242427)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP114] to MUM1 - BSA and Azide free
  • Suitable for: Flow Cyt, WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-MUM1 antibody [SP114] - BSA and Azide free
    See all MUM1 primary antibodies

  • Description

    Rabbit monoclonal [SP114] to MUM1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow, Dog

  • Immunogen

    Recombinant fragment within Human MUM1 aa 100 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q15306

  • Positive control

    • IHC-P: Hodgkins lymphoma, HK lymphoma, " Human Hodgkins lymphoma", and Human colon tissue; Flow Cyt: Ramos cells.

  • General notes

    FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    ab242427 is the carrier-free version of ab227646. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab242427 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUM1 antibody [SP114] - BSA and Azide free (ab242427)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUM1 antibody [SP114] - BSA and Azide free (ab242427)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling MUM1 with ab227646 at 1/100 dilution (2.32 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227646)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUM1 antibody [SP114] - BSA and Azide free (ab242427)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUM1 antibody [SP114] - BSA and Azide free (ab242427)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human Hodgkin's lymphoma tissue sections labeling MUM1 with ab227646 at 1/100 dilution (2.32 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227646)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUM1 antibody [SP114] - BSA and Azide free (ab242427)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUM1 antibody [SP114] - BSA and Azide free (ab242427)

    Formalin-fixed, paraffin-embedded human Hodgkin's lymphoma tissue stained for MUM1 using ab227646 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab227646).

  • Flow Cytometry - Anti-MUM1 antibody [SP114] - BSA and Azide free (ab242427)
    Flow Cytometry - Anti-MUM1 antibody [SP114] - BSA and Azide free (ab242427)
    Flow Cytometry analysis of Ramos(Human Burkitt's lymphoma B lymphocyte) cells labeling MUM1 with purified ab227646 at 1:230 dilution (1.01 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab115803).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUM1 antibody [SP114] - BSA and Azide free (ab242427)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUM1 antibody [SP114] - BSA and Azide free (ab242427)

    Formalin-fixed, paraffin-embedded human HK lymphoma tissue stained for MUM1 using ab227646 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab227646).

  • Anti-MUM1 antibody [SP114] - BSA and Azide free (ab242427)
    Anti-MUM1 antibody [SP114] - BSA and Azide free (ab242427)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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