Anti-Cytochrome P450 Reductase antibody (ab227071)
Key features and details
- Rabbit polyclonal to Cytochrome P450 Reductase
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Cytochrome P450 Reductase antibody
See all Cytochrome P450 Reductase primary antibodies -
Description
Rabbit polyclonal to Cytochrome P450 Reductase -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Guinea pig, Cow, Dog, Pig, Rhesus monkey -
Immunogen
Recombinant fragment within Human Cytochrome P450 Reductase (N terminal). The exact sequence is proprietary.
Database link: P16435 -
Positive control
- WB: HeLa cell extract; Mouse lung lysate. ICC/IF: HeLa cells. IHC-P: Human ovarian carcinoma tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Cytochrome P450 Reductase antibody (ab227071) at 1/500 dilution
Lane 1 : Wild-type HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 2 : Cytochrome P450 Reductase knock out HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 76 kDa7.5% SDS-PAGE gel.
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Anti-Cytochrome P450 Reductase antibody (ab227071) at 1/500 dilution + Mouse lung lysate at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 76 kDa7.5% SDS-PAGE gel.
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Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Cytochrome P450 Reductase (green) using ab227071 at 1/200 dilution in ICC/IF.
Lower panel: Costained with Hoechst 33342.
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Paraffin-embedded human ovarian carcinoma tissue stained for Cytochrome P450 Reductase using ab227071 at 1/500 dilution in immunohistochemical analysis.