Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Bub3 antibody [EPR5319(2)] (ab133699)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5319(2)] to Bub3
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-Bub3 antibody [EPR5319(2)]
    See all Bub3 primary antibodies

  • Description

    Rabbit monoclonal [EPR5319(2)] to Bub3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    A synthetic peptide corresponding to residues near the N terminus of Human Bub3 (UniProt ID: O43684)

  • Positive control

    • WB: Lysates of Jurkat, HeLa, 293T IHC-P: Human breast tissue, Human thyroid carcinoma tissue ICC/IF: 293T cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR5319(2)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Bub3 antibody [EPR5319(2)] (ab133699)
    Western blot - Anti-Bub3 antibody [EPR5319(2)] (ab133699)
    All lanes : Anti-Bub3 antibody [EPR5319(2)] (ab133699) at 1/10000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled Goat anti Rabbit at 1/2000 dilution

    Predicted band size: 37 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-Bub3 antibody [EPR5319(2)] (ab133699)
    Immunocytochemistry/ Immunofluorescence - Anti-Bub3 antibody [EPR5319(2)] (ab133699)

    Immunocytochemistry/Immunofluorescence analysis of HepG2 (human hepatocellular carcinoma) labelling Bub3 with purified ab133699 at 1/2000. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

    Control: PBS only

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bub3 antibody [EPR5319(2)] (ab133699)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bub3 antibody [EPR5319(2)] (ab133699)

    Immunohistochemical analysis of paraffin-embedded human breast tissue labelling Bub3 using ab133699 at 1/100 dilution

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bub3 antibody [EPR5319(2)] (ab133699)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bub3 antibody [EPR5319(2)] (ab133699)

    Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue labelling Bub3 using ab133699 at 1/100 dilution

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Bub3 antibody [EPR5319(2)] (ab133699)
    Immunocytochemistry/ Immunofluorescence - Anti-Bub3 antibody [EPR5319(2)] (ab133699)
    Immunofluorescent staining Bub3 in 293T cells using ab133699 at 1/250 dilution
  • Anti-Bub3 antibody [EPR5319(2)] (ab133699)
    Anti-Bub3 antibody [EPR5319(2)] (ab133699)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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