Abcam’s Enterokinase cleavage kit (ab207001) efficiently remove tags from recombinant fusion proteins containing accessible enteropeptidase-specific recognition sequence.

Enteropeptidase (enterokinase, EC 3.4.21.9) is a serine protease involved in activation of trypsinogen to trypsin. It recognizes a highly specific amino acid sequence ‘DDDDK’ and cleaves after the lysine (K) residue. The high specific activity of enteropeptidase is also used to cleave native and fusion proteins containing this recognition motif, and is often used to remove the tag from a recombinant protein. Abcam’s Enterokinase cleavage kit contains a highly active light chain fragment of human enteropeptidase. Our pure enzyme is an excellent tool to obtain a wild type protein sequence from a fusion protein that contains the enteropeptidase recognition sequence. This kit is sufficient for cleaving at least 5 mg of target protein. The residual enteropeptidase left in the reaction mix will not interfere with most experiments that the target protein will be used in. Following cleavage of the target protein, the enteropeptidase may be removed using nickel or cobalt beads if required.