Anti-Dishevelled 2 antibody (ab104438)
Key features and details
- Rabbit polyclonal to Dishevelled 2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Dishevelled 2 antibody
See all Dishevelled 2 primary antibodies -
Description
Rabbit polyclonal to Dishevelled 2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide, corresponding to a region within amino acids 650-700 of Human Dishevelled 2 (NP_004413.1).
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Positive control
- HeLa and 293T whole cell lysates
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Dishevelled 2 antibody (ab104438)All lanes : Anti-Dishevelled 2 antibody (ab104438) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 79 kDa
Exposure time: 3 minutes
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Immunoprecipitation - Anti-Dishevelled 2 antibody (ab104438)Detection of Dishevelled 2 in immunoprecipitates of HeLa whole cell lysate (1 mg lysate for IP, 20% of IP loaded on gel) using ab104438 at 3 µg/mg lysate for IP, and at 1 µg/ml for the subsequent western blot.
Detection: chemiluminescence with exposure time of 30 seconds.
