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Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (ab124933)

Price and availability

251 280 ₸

Availability

Order now and get it on Thursday March 04, 2021

Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (ab124933)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPNCIR145] to Dishevelled 2 (phospho S143)
  • Suitable for: WB
  • Reacts with: Mouse, Human

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Overview

  • Product name

    Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145]
    See all Dishevelled 2 primary antibodies
  • Description

    Rabbit monoclonal [EPNCIR145] to Dishevelled 2 (phospho S143)
  • Host species

    Rabbit
  • Specificity

    ab124933 only detects Dishevelled 2 phosphorylated at Serine 143.
  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Dishevelled 2 (phospho S143).
    (Peptide available as ab173138)

  • Positive control

    • WB: F9 and HEK293 cell lysates.
  • General notes

    This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Kyung Lee. View antibodies from NCI Center for Cancer Research Collaboration.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPNCIR145
  • Isotype

    IgG
  • Research areas

    • Stem Cells
    • Signaling Pathways
    • Wnt
    • Cytoplasmic
    • Stem Cells
    • Signaling Pathways
    • Notch
    • Cytoplasmic

Images

  • Western blot - Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (ab124933)
    Western blot - Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (ab124933)
    All lanes : Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (ab124933) at 1/1000 dilution (purified)

    Lane 1 : Untreated F9 cell lysate
    Lane 2 : Alkaline phosphatase treated F9 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 78 kDa
    Observed band size: 95 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (ab124933)
    Western blot - Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (ab124933)
    All lanes : Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (ab124933) at 1/5000 dilution (purified)

    Lane 1 : Untreated HEK293 cell lysate
    Lane 2 : Alkaline phosphatase treated HEK293 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 78 kDa
    Observed band size: 95 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (ab124933)
    Western blot - Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (ab124933)
    All lanes : Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (ab124933) at 1/1000 dilution (unpurified)

    Lane 1 : F9 cell lysate untreated
    Lane 2 : F9 cell lysate treated with Alkaline Phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 78 kDa
    Observed band size: 95 kDa why is the actual band size different from the predicted?

  • Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (ab124933)
    Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (ab124933)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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