Anti-Fast Myosin Skeletal Heavy chain antibody (ab91506)
Key features and details
- Rabbit polyclonal to Fast Myosin Skeletal Heavy chain
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Fast Myosin Skeletal Heavy chain antibody
See all Fast Myosin Skeletal Heavy chain primary antibodies -
Description
Rabbit polyclonal to Fast Myosin Skeletal Heavy chain -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Sheep, Guinea pig, Cow, Pig
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Immunogen
Synthetic peptide corresponding to Human Fast Myosin Skeletal Heavy chain aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab102066) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab91506 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 223 kDa (predicted molecular weight: 223 kDa).IHC-P (1) Use a concentration of 1 µg/ml.Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 223 kDa (predicted molecular weight: 223 kDa).IHC-P
Use a concentration of 1 µg/ml.Target
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Function
Muscle contraction. -
Sequence similarities
Contains 1 IQ domain.
Contains 1 myosin head-like domain. -
Domain
The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2). -
Cellular localization
Cytoplasm > myofibril. Thick filaments of the myofibrils. - Information by UniProt
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Database links
- Entrez Gene: 281337 Cow
- Entrez Gene: 4619 Human
- Entrez Gene: 17879 Mouse
- Entrez Gene: 287408 Rat
- Omim: 160730 Human
- Omim: 160740 Human
- SwissProt: Q9BE40 Cow
- SwissProt: P12882 Human
see all -
Alternative names
- adult 1 antibody
- MYH1 antibody
- MYH1_HUMAN antibody
see all
Images
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Western blot - Anti-Fast Myosin Skeletal Heavy chain antibody (ab91506)All lanes : Anti-Fast Myosin Skeletal Heavy chain antibody (ab91506) at 1 µg/ml
Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate
Lane 3 : Skeletal Muscle (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 223 kDa
Observed band size: 223 kDa
Exposure time: 1 minuteThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab91506 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fast Myosin Skeletal Heavy chain antibody (ab91506)Image courtesy of Mr Carl HobbsFormalin-fixed, paraffin-embedded pig skeletal muscle tissue stained for Fast Myosin Skeletal Heavy chain using ab91506 at 1/1000 dilution (for 2 hours at room temperature) in immunohistochemical analysis (upper image).
Note: Lower image is a comparison using ab51263.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fast Myosin Skeletal Heavy chain antibody (ab91506)IHC image of Fast Myosin Skeletal Heavy chain staining in Mouse skeletal muscle FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab91506, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (39)
ab91506 has been referenced in 39 publications.
- Zheng J et al. Pterostilbene Enhances Endurance Capacity via Promoting Skeletal Muscle Adaptations to Exercise Training in Rats. Molecules 25:N/A (2020). PubMed: 31906449
- Wang J & Wu T Testosterone improves muscle function of the extensor digitorum longus in rats with sepsis. Biosci Rep 40:N/A (2020). PubMed: 31967292
- Srikuea R et al. Regulation of vitamin D system in skeletal muscle and resident myogenic stem cell during development, maturation, and ageing. Sci Rep 10:8239 (2020). PubMed: 32427932
- He J et al. Transcriptome Characterization of Repressed Embryonic Myogenesis Due to Maternal Calorie Restriction. Front Cell Dev Biol 8:527 (2020). PubMed: 32671071
- Rodriguez BL et al. A tissue engineering approach for repairing craniofacial volumetric muscle loss in a sheep following a 2, 4, and 6-month recovery. PLoS One 15:e0239152 (2020). PubMed: 32956427
Images
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Western blot - Anti-Fast Myosin Skeletal Heavy chain antibody (ab91506)All lanes : Anti-Fast Myosin Skeletal Heavy chain antibody (ab91506) at 1 µg/ml
Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate
Lane 3 : Skeletal Muscle (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 223 kDa
Observed band size: 223 kDa
Exposure time: 1 minuteThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab91506 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fast Myosin Skeletal Heavy chain antibody (ab91506) Image courtesy of Mr Carl Hobbs
Formalin-fixed, paraffin-embedded pig skeletal muscle tissue stained for Fast Myosin Skeletal Heavy chain using ab91506 at 1/1000 dilution (for 2 hours at room temperature) in immunohistochemical analysis (upper image).
Note: Lower image is a comparison using ab51263.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fast Myosin Skeletal Heavy chain antibody (ab91506)IHC image of Fast Myosin Skeletal Heavy chain staining in Mouse skeletal muscle FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab91506, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
