Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Fast Myosin Skeletal Heavy chain antibody (ab91506)

Key features and details

  • Rabbit polyclonal to Fast Myosin Skeletal Heavy chain
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Fast Myosin Skeletal Heavy chain antibody
    See all Fast Myosin Skeletal Heavy chain primary antibodies

  • Description

    Rabbit polyclonal to Fast Myosin Skeletal Heavy chain

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Guinea pig, Cow, Pig

  • Immunogen

    Synthetic peptide corresponding to Human Fast Myosin Skeletal Heavy chain aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab102066)

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab91506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 223 kDa (predicted molecular weight: 223 kDa).
IHC-P (1)
Use a concentration of 1 µg/ml.
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 223 kDa (predicted molecular weight: 223 kDa).
IHC-P
Use a concentration of 1 µg/ml.

Target

  • Function

    Muscle contraction.

  • Sequence similarities

    Contains 1 IQ domain.
    Contains 1 myosin head-like domain.

  • Domain

    The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
    Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).

  • Cellular localization

    Cytoplasm > myofibril. Thick filaments of the myofibrils.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • adult 1 antibody
    • MYH1 antibody
    • MYH1_HUMAN antibody
    • MyHC-2x antibody
    • MyHC-IIx/d antibody
    • Myosin heavy chain 1 antibody
    • Myosin heavy chain 2x antibody
    • Myosin heavy chain antibody
    • Myosin heavy chain IIx/d antibody
    • Myosin-1 antibody
    • skeletal muscle antibody
    see all

Images

  • Western blot - Anti-Fast Myosin Skeletal Heavy chain antibody (ab91506)
    Western blot - Anti-Fast Myosin Skeletal Heavy chain antibody (ab91506)
    All lanes : Anti-Fast Myosin Skeletal Heavy chain antibody (ab91506) at 1 µg/ml

    Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate
    Lane 3 : Skeletal Muscle (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 223 kDa
    Observed band size: 223 kDa


    Exposure time: 1 minute


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab91506 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fast Myosin Skeletal Heavy chain antibody (ab91506)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fast Myosin Skeletal Heavy chain antibody (ab91506)Image courtesy of Mr Carl Hobbs

    Formalin-fixed, paraffin-embedded pig skeletal muscle tissue stained for Fast Myosin Skeletal Heavy chain using ab91506 at 1/1000 dilution (for 2 hours at room temperature) in immunohistochemical analysis (upper image).

    Note: Lower image is a comparison using ab51263.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fast Myosin Skeletal Heavy chain antibody (ab91506)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fast Myosin Skeletal Heavy chain antibody (ab91506)
    IHC image of Fast Myosin Skeletal Heavy chain staining in Mouse skeletal muscle FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab91506, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX

References (39)

Publishing research using ab91506? Please let us know so that we can cite the reference in this datasheet.

ab91506 has been referenced in 39 publications.

  • Zheng J  et al. Pterostilbene Enhances Endurance Capacity via Promoting Skeletal Muscle Adaptations to Exercise Training in Rats. Molecules 25:N/A (2020). PubMed: 31906449
  • Wang J & Wu T Testosterone improves muscle function of the extensor digitorum longus in rats with sepsis. Biosci Rep 40:N/A (2020). PubMed: 31967292
  • Srikuea R  et al. Regulation of vitamin D system in skeletal muscle and resident myogenic stem cell during development, maturation, and ageing. Sci Rep 10:8239 (2020). PubMed: 32427932
  • He J  et al. Transcriptome Characterization of Repressed Embryonic Myogenesis Due to Maternal Calorie Restriction. Front Cell Dev Biol 8:527 (2020). PubMed: 32671071
  • Rodriguez BL  et al. A tissue engineering approach for repairing craniofacial volumetric muscle loss in a sheep following a 2, 4, and 6-month recovery. PLoS One 15:e0239152 (2020). PubMed: 32956427
View all Publications for this product

Images

  • Western blot - Anti-Fast Myosin Skeletal Heavy chain antibody (ab91506)
    Western blot - Anti-Fast Myosin Skeletal Heavy chain antibody (ab91506)
    All lanes : Anti-Fast Myosin Skeletal Heavy chain antibody (ab91506) at 1 µg/ml

    Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate
    Lane 3 : Skeletal Muscle (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 223 kDa
    Observed band size: 223 kDa


    Exposure time: 1 minute


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab91506 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fast Myosin Skeletal Heavy chain antibody (ab91506)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fast Myosin Skeletal Heavy chain antibody (ab91506) Image courtesy of Mr Carl Hobbs

    Formalin-fixed, paraffin-embedded pig skeletal muscle tissue stained for Fast Myosin Skeletal Heavy chain using ab91506 at 1/1000 dilution (for 2 hours at room temperature) in immunohistochemical analysis (upper image).

    Note: Lower image is a comparison using ab51263.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fast Myosin Skeletal Heavy chain antibody (ab91506)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fast Myosin Skeletal Heavy chain antibody (ab91506)
    IHC image of Fast Myosin Skeletal Heavy chain staining in Mouse skeletal muscle FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab91506, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX

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