Anti-NUP88 antibody (ab79785)
Key features and details
- Rabbit polyclonal to NUP88
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NUP88 antibody
See all NUP88 primary antibodies -
Description
Rabbit polyclonal to NUP88 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human NUP88 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab90188) -
Positive control
- This antibody gave a positive signal in HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab79785 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatCowOrangutanApplication Abreviews Notes WB (2) Use a concentration of 1 µg/ml. Detects a band of approximately 87 kDa (predicted molecular weight: 83 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 87 kDa (predicted molecular weight: 83 kDa).Target
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Function
Essential component of nuclear pore complex. -
Tissue specificity
Ubiquitous. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus > nuclear pore complex. - Information by UniProt
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Database links
- Entrez Gene: 4927 Human
- Entrez Gene: 19069 Mouse
- Entrez Gene: 113929 Rat
- Omim: 602552 Human
- SwissProt: Q99567 Human
- SwissProt: Q8CEC0 Mouse
- SwissProt: O08658 Rat
- Unigene: 584784 Human
see all -
Alternative names
- 88 kDa nucleoporin antibody
- karyoporin antibody
- Nuclear pore complex protein 88 antibody
see all
Images
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Western blot - Anti-NUP88 antibody (ab79785)Anti-NUP88 antibody (ab79785) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 87 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
Nuclear pore complex protein Nup88 contains a number of potential phosphorylation sites (SwissProt) which may explain its migration at a higher molecular weight (87 kDa) than predicted (83 kDa).
Datasheets and documents
References (4)
ab79785 has been referenced in 4 publications.
- Fichtman B et al. Pathogenic Variants in NUP214 Cause "Plugged" Nuclear Pore Channels and Acute Febrile Encephalopathy. Am J Hum Genet 105:48-64 (2019). PubMed: 31178128
- Zhang K et al. Stress Granule Assembly Disrupts Nucleocytoplasmic Transport. Cell 173:958-971.e17 (2018). PubMed: 29628143
- Grima JC et al. Mutant Huntingtin Disrupts the Nuclear Pore Complex. Neuron 94:93-107.e6 (2017). PubMed: 28384479
- Sugiyama K et al. Calpain-Dependent Degradation of Nucleoporins Contributes to Motor Neuron Death in a Mouse Model of Chronic Excitotoxicity. J Neurosci 37:8830-8844 (2017). PubMed: 28821644
Images
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Western blot - Anti-NUP88 antibody (ab79785)Anti-NUP88 antibody (ab79785) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 87 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
Nuclear pore complex protein Nup88 contains a number of potential phosphorylation sites (SwissProt) which may explain its migration at a higher molecular weight (87 kDa) than predicted (83 kDa).
