Anti-CUL-4A + CUL-4B antibody [EPR3200] - BSA and Azide free (ab247477)
![Anti-CUL-4A + CUL-4B antibody [EPR3200] - BSA and Azide free (ab247477)](https://www.abcam.com/ps/products/247/ab247477/Images/ab247477-403253-anti-cul-4a-cul-4b-antibody-epr3200-bsa-and-azide-free-western-blot.jpg "Anti-CUL-4A + CUL-4B antibody [EPR3200] - BSA and Azide free (ab247477)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3200] to CUL-4A + CUL-4B - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-CUL-4A + CUL-4B antibody [EPR3200] - BSA and Azide free
See all CUL-4A + CUL-4B primary antibodies -
Description
Rabbit monoclonal [EPR3200] to CUL-4A + CUL-4B - BSA and Azide free -
Host species
Rabbit -
Specificity
Due to the close homology between Cullin 4A and 4B this antibody is likely to react with both forms.
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Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab247477 is the carrier-free version of ab76470 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab247477 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as Cullin 4a, Cullin 4A/B
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR3200 -
Isotype
IgG
Images
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Western blot - Anti-CUL-4A + CUL-4B antibody [EPR3200] - BSA and Azide free (ab247477)All lanes : Anti-CUL-4A + CUL-4B antibody [EPR3200] (ab76470) at 1/5000 dilution
Lane 1 : Fetal uterus lysate
Lane 2 : HeLa lysate
Lane 3 : A375 lysate
Lane 4 : Fetal testis lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 102 kDa
Observed band size: 102,88 kDa why is the actual band size different from the predicted?This data was developed using ab76470, the same antibody clone in a different buffer formulation.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CUL-4A + CUL-4B antibody [EPR3200] - BSA and Azide free (ab247477)](https://www.abcam.com/ps/products/247/ab247477/Images/ab247477-1-anti-cul-4a-cul-4b-antibody-epr3200-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CUL-4A + CUL-4B antibody [EPR3200] - BSA and Azide free (ab247477) -
![Anti-CUL-4A + CUL-4B antibody [EPR3200] - BSA and Azide free (ab247477)](https://www.abcam.com/ps/products/247/ab247477/Images/ab247477-3-benefits-of-recombinant-antibodies.png)
Anti-CUL-4A + CUL-4B antibody [EPR3200] - BSA and Azide free (ab247477)