Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TPMT antibody [EPR10820(B)] - BSA and Azide free (ab249198)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10820(B)] to TPMT - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), IHC-P, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-TPMT antibody [EPR10820(B)] - BSA and Azide free
    See all TPMT primary antibodies

  • Description

    Rabbit monoclonal [EPR10820(B)] to TPMT - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab249198 is the carrier-free version of ab155284.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-TPMT antibody [EPR10820(B)] - BSA and Azide free (ab249198)
    Western blot - Anti-TPMT antibody [EPR10820(B)] - BSA and Azide free (ab249198)

    This data was developed using ab155284, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)

    Lane 2: TPMT knockout HAP1 whole cell lysate (20 µg)

    Lane 3: HepG2 whole cell lysate (20 µg)

    Lane 4: Jurkat whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab155284 observed at 28 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab155284 was shown to recognize TPMT in wild-type HAP1 cells as signal was lost at the expected MW in TPMT knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and TPMT knockout samples were subjected to SDS-PAGE. ab155284 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 2 µg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-TPMT antibody [EPR10820(B)] - BSA and Azide free (ab249198)
    Western blot - Anti-TPMT antibody [EPR10820(B)] - BSA and Azide free (ab249198)
    All lanes : Anti-TPMT antibody [EPR10820(B)] (ab155284) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : TF1 cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 28 kDa



    This data was developed using ab155284, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] - BSA and Azide free (ab249198)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] - BSA and Azide free (ab249198)

    This data was developed using ab155284, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling TPMT with ab155284 at 1/100 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] - BSA and Azide free (ab249198)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] - BSA and Azide free (ab249198)
    This data was developed using ab155284, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling TPMT with ab155284 at 1/100 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Flow Cytometry - Anti-TPMT antibody [EPR10820(B)] - BSA and Azide free (ab249198)
    Flow Cytometry - Anti-TPMT antibody [EPR10820(B)] - BSA and Azide free (ab249198)
    This data was developed using ab155284, the same antibody clone in a different buffer formulation.Flow cytometric analysis of permeabilized K562 cells labeling TPMT with ab155284 at 1/100 dilution (red) compared with a rabbit IgG (negative) (green).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] - BSA and Azide free (ab249198)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] - BSA and Azide free (ab249198)
    This data was developed using ab155284, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human normal uterus tissue using ab155284 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] - BSA and Azide free (ab249198)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] - BSA and Azide free (ab249198)
    This data was developed using ab155284, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human gastric adenocarcinoma tissue using ab155284 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] - BSA and Azide free (ab249198)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] - BSA and Azide free (ab249198)
    This data was developed using ab155284, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab155284 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Anti-TPMT antibody [EPR10820(B)] - BSA and Azide free (ab249198)
    Anti-TPMT antibody [EPR10820(B)] - BSA and Azide free (ab249198)

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