Thiazolyl blue tetrazolium bromide (MTT), membrane-permeable dye (ab146345)
Key features and details
- Membrane-permeable dye for assessing cell viability and proliferation
- CAS Number: 298-93-1
- Soluble in water to 5 mg/ml
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
Thiazolyl blue tetrazolium bromide (MTT), membrane-permeable dye -
Description
Membrane-permeable dye for assessing cell viability and proliferation -
Alternative names
- 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide
- Methylthiazolyldiphenyl-tetrazolium bromide
- MTT
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Biological description
Membrane-permeable dye used in measurement of cell proliferation. It produces a yellowish tetrazole solution that is converted to dark blue, water-insoluble MTT formazan by intracellular NAD(P)H-oxidoreductases of living cells. The blue crystals are solubilized and the intensity is measured colorimetrically at 570 nm.
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CAS Number
298-93-1 -
Chemical structure
Properties
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Emission
570nm -
Chemical name
2-(4,5-Dimethyl-2-thiazolyl)-3,5-diphenyl-2H-tetrazolium bromide -
Molecular weight
414.33 -
Molecular formula
C18H16BrN5S -
Storage instructions
Store at +4°C. The product can be stored for up to 12 months. -
Solubility overview
Soluble in water to 5 mg/ml -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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Source
Synthetic
Images
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Functional Studies - Thiazolyl blue tetrazolium bromide (MTT), membrane-permeable dye (ab146345) Dong et al PLoS One. 2015; 10(8): e0136051. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
NaHS protects viability of HEI-OC1 cells upon gentamicin toxicity.
Gentamicin (GM) was added to the culture medium to a concentration of 0 (control), 1, 2.5 and 5 mM respectively incubated for 48 hours. Representative culture images (A) and viability measured by MTT assay (B) of HEI-OC1 cell culture after gentamicin (GM) treatment were shown.
Scale bar 100 μm. Values were represented as the mean ± SEM from three independent experiments. * P
(After Figure 1A & B of Dong et al).
