Anti-PDP2 antibody [MS41A 3C3BC10] (ab133982)
Key features and details
- Mouse monoclonal [MS41A 3C3BC10] to PDP2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-PDP2 antibody [MS41A 3C3BC10]
See all PDP2 primary antibodies -
Description
Mouse monoclonal [MS41A 3C3BC10] to PDP2 -
Host species
Mouse -
Specificity
ab133982 does not react with Human Recombinant PDP1 or Human Recombinant PDK. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Full length protein. This information is considered to be commercially sensitive.
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Positive control
- Recombinant PDP2 protein, Human heart or liver homogenate, HeLa, HEK293, HepG2 and jurkat whole cell lysate.
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General notes
This antibody clone is manufactured by Abcam.
Product was previously marketed under the MitoSciences sub-brand.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99% HEPES buffered saline -
Concentration information loading...
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Purity
Ammonium Sulphate Precipitation -
Purification notes
Near homogeneity as judged by SDS-PAGE. ab133982 was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by ammonium sulfate precipitation. -
Clonality
Monoclonal -
Clone number
MS41A 3C3BC10 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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All lanes : Anti-PDP2 antibody [MS41A 3C3BC10] (ab133982) at 1 µg/ml
Lane 1 : Molecular weight marker
Lane 2 : Recombinant human PDP2 protein (ab110358) at 0.01 µg
Lane 3 : Recombinant Human PDP1/PDP protein (His tag) (ab110357) at 0.01 µg
Lane 4 : Recombinant human PDK1 protein (ab110359) at 0.01 µg
Lane 5 : HHH (Human heart homogenate) at 20 µg
Lane 6 : HLH (Human liver homogenate) at 20 µg
Lane 7 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 8 : HEK293 (Human embryonic kidney 293 cell line) Whole Cell Lysate at 10 µg
Lane 9 : HepG2 (Human hepatocellular carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 10 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Secondary
All lanes : Goat polyclonal to Mouse IgG – H&L – Pre-Adsorbed (HRP) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 60 kDa
Exposure time: 5 minutes
Predicted mature band size: 52 kDa
Observed band size: 52 kDa
Note: The antibody recognizes both the mature (52 kDa) and the immature (60 kDa) versions of PDP2 protein. The immunogen was mature PDP2 protein, lacking the first 66 amino acids which are used as a transit peptide for mitochondrial targeting. The antibody produces a band against the endogenous protein at the mature weight in tissue homogenates, but not in cancer cells where the protein may fail to mature.