Annexin V-APC Assay Kit (ab236215)
Key features and details
- Assay type: Cell-based (quantitative)
- Detection method: Fluorescent
- Platform: Microplate reader, Flow cytometer
- Sample type: Suspension cells
Overview
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Product name
Annexin V-APC Assay Kit
See all Annexin V/ANXA5 kits -
Detection method
Fluorescent -
Sample type
Suspension cells -
Assay type
Cell-based (quantitative) -
Species reactivity
Reacts with: Human -
Product overview
Annexin V-APC Assay Kit (ab236215) employs an APC-conjugated Annexin V as a probe for phosphatidylserine on the outer membrane of apoptotic cells. DAPI is used as a marker of cell membrane permeability seen in very late apoptotic or necrotic cells. The reagents provided are sufficient to run 100 samples when using a 96-well format.
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Platform
Microplate reader, Flow cytometer
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 100 tests Annexin V APC Assay Reagent 1 x 100 tests Cell-Based Assay Annexin V Binding Buffer (10X) 1 x 50ml DAPI Viability Dye 1 x 100µl -
Research areas
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Function
This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. -
Involvement in disease
Pregnancy loss, recurrent, 3 -
Sequence similarities
Belongs to the annexin family.
Contains 4 annexin repeats. -
Domain
The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
Post-translational
modificationsS-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. - Information by UniProt
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Alternative names
- Anchorin CII
- Annexin 5
- Annexin A5
see all -
Database links
- Entrez Gene: 308 Human
- Omim: 131230 Human
- SwissProt: P08758 Human
- Unigene: 480653 Human
Images
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Ultraviolet light induces apoptosis in Jurkat cells.
Jurkat (Human T cell leukemia cell line from peripheral blood) cells were left untreated (panel A) or stimulated with 200 mJ/cm2 ultraviolet light (panel B) and then incubated for 4 hours at 37°C. Cells were stained using Annexin V APC Assay Reagent at a 1:200 dilution in 1X Binding Buffer for 15 minutes at RT in the dark. After centrifugation and removal of the supernatant, cells were resuspended in 1:100 DAPI in PBS.
