Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BCMA antibody [EPRBOB-R1-F1-24] (ab245940)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPRBOB-R1-F1-24] to BCMA
  • Suitable for: WB, ICC/IF, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-BCMA antibody [EPRBOB-R1-F1-24]
    See all BCMA primary antibodies

  • Description

    Rabbit monoclonal [EPRBOB-R1-F1-24] to BCMA

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: U266B1 cells. WB: U266B1 whole cell lysate. IP: U266B1 whole cell lysate. Flow Cyt: U266B1 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-BCMA antibody [EPRBOB-R1-F1-24] (ab245940)
    Immunocytochemistry/ Immunofluorescence - Anti-BCMA antibody [EPRBOB-R1-F1-24] (ab245940)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U266B1 (human multiple myeloma B lymphocyte) cells labeling BCMA with ab245940 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in U266B1 cell line. Negative control cells: Jurkat (PMID: 11104810. The nuclear counterstain is DAPI (blue).
    Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red). The negative control is the secondary antibody only.

  • Western blot - Anti-BCMA antibody [EPRBOB-R1-F1-24] (ab245940)
    Western blot - Anti-BCMA antibody [EPRBOB-R1-F1-24] (ab245940)
    All lanes : Anti-BCMA antibody [EPRBOB-R1-F1-24] (ab245940) at 1/300 dilution

    Lane 1 : Jurkat (human T cell leukemia T lymphocyte) whole cell lysate
    Lane 2 : U266B1 (human multiple myeloma B lymphocyte) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 20 kDa
    Observed band size: 19,23-30 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 23776238).

    Negative control: Jurkat (PMID: 11104810).

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-BCMA antibody [EPRBOB-R1-F1-24] (ab245940)
    Immunoprecipitation - Anti-BCMA antibody [EPRBOB-R1-F1-24] (ab245940)

    BCMA was immunoprecipitated from 0.35 mg U266B1 (human multiple myeloma B lymphocyte) whole cell lysate with ab245940 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab245940 at 1/300 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.

    Lane 1: U266B1 whole cell lysate 10 µg (Input).
    Lane 2: ab245940 IP in U266B1 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab245940 in U266B1 whole cell lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 30 seconds.

  • Flow Cytometry - Anti-BCMA antibody [EPRBOB-R1-F1-24] (ab245940)
    Flow Cytometry - Anti-BCMA antibody [EPRBOB-R1-F1-24] (ab245940)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized U266B1 (human multiple myeloma B lymphocyte) cell line labeling BCMA with ab245940 at 1/60 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

  • Anti-BCMA antibody [EPRBOB-R1-F1-24] (ab245940)
    Anti-BCMA antibody [EPRBOB-R1-F1-24] (ab245940)

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