Annexin V/ANXA5-FITC Apoptosis Detection Reagent (500X) (ab14082)
Overview
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Product name
Annexin V/ANXA5-FITC Apoptosis Detection Reagent (500X)
See all Annexin V/ANXA5 reagents -
Tested applications
Suitable for: FM, Flow Cyt, ICC/IFmore details -
General notes
Annexin V/ANXA5-FITC Apoptosis Detection Reagent ab14082 can be used in a 10 min, one-step staining procedure to detect apoptosis by staining phosphatidylserine molecules which have translocated to the outside of the cell membrane. Analysis is by flow cytometry or fluorescence microscopy.
This Annexin V/ANXA5-FITC reagent can be used with Annexin V/ANXA5 binding buffer ab14084 (see protocol section for instructions). The Annexin V/ANXA5-FITC reagent is also available as part of Annexin V/ANXA5-FITC apoptosis kit ab14085.
For more apoptosis assays, review the full set of Annexin V/ANXA5 assays, or the apoptosis assay and apoptosis marker guide.
Properties
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Form
Liquid -
Storage instructions
Store at +4°C. Do not freeze. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Research areas
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Function
This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. -
Involvement in disease
Pregnancy loss, recurrent, 3 -
Sequence similarities
Belongs to the annexin family.
Contains 4 annexin repeats. -
Domain
The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
Post-translational
modificationsS-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. - Information by UniProt
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Alternative names
- Anchorin CII
- Annexin 5
- Annexin A5
see all
Images
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Flow Cytometry - Annexin V/ANXA5-FITC Apoptosis Detection Reagent (500X) (ab14082) Image from Harper MT et al, J Biol Chem. 2010 Jun 25;285(26):19865-73. Epub 2010 Apr 13. DOI 10.1074/jbc.M109.085654
Murine platelets were stimulated with CRP (5 μg/ml) in the presence of annexin V-fluorescein isothiocyanate for 10 minutes. The annexin V-positive population is indicated (AnV +ve).
