Anti-delta 1 Catenin/CAS (phospho Y280) antibody [EPR2384(2)] (ab173578)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2384(2)] to delta 1 Catenin/CAS (phospho Y280)
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Overview
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Product name
Anti-delta 1 Catenin/CAS (phospho Y280) antibody [EPR2384(2)]
See all delta 1 Catenin/CAS primary antibodies -
Description
Rabbit monoclonal [EPR2384(2)] to delta 1 Catenin/CAS (phospho Y280) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanIP HumanWB Human -
Immunogen
Synthetic peptide within Human delta 1 Catenin/CAS aa 250-350 (phospho Y280) (Cysteine residue). The exact sequence is proprietary.
Database link: O60716 -
Positive control
- Pervanadate treated A431 cell lysate, Human gastric adenocarcinoma
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Do Not Freeze. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR2384(2) -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-delta 1 Catenin/CAS (phospho Y280) antibody [EPR2384(2)] (ab173578) at 1/50000 dilution
Lane 1 : Untreated A431 cell lysate
Lane 2 : Pervanadate treated A431 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 90-120 kDa
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Western blot analysis on immunoprecipitation pellet from A431 cells treated with pervanadate lysate: ab173578 at 1/10 dilution immunoprecipitated delta 1 Catenin/CAS (phospho Y280).
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Immunohistochemical analysis of paraffin-embedded Human gastric adenocarcinoma tissue labeling delta 1 Catenin/CAS (phospho Y280) with ab173578 at 1/50 dilution
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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