Antibodies, Proteins, Kits and Reagents for Life Science

341 740 ₸ Product size

100 µl 341 740 ₸ 10 µl 137 366 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Tropomyosin 1 (alpha) antibody [EPR5159] (ab133292)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR5159] to Tropomyosin 1 (alpha)
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-Tropomyosin 1 (alpha) antibody [EPR5159]
See all Tropomyosin 1 (alpha) primary antibodies
Description

Rabbit monoclonal [EPR5159] to Tropomyosin 1 (alpha)
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human Tropomyosin 1 (alpha) aa 250-350 (C terminal). The exact sequence is proprietary.
Positive control
- Human skeletal muscle, Human heart, HUVEC, C6, RAW 264.7, PC12 and NIH 3T3 cell lysates; Human muscle tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR5159
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tropomyosin 1 (alpha) antibody [EPR5159] (ab133292)

Imunohistochemical analysis of paraffin-embedded Human muscle tissue labelling Tropomyosin 1 (alpha) with ab133292 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot - Anti-Tropomyosin 1 (alpha) antibody [EPR5159] (ab133292)

All lanes : Anti-Tropomyosin 1 (alpha) antibody [EPR5159] (ab133292) at 1/1000 dilution

Lane 1 : Human skeletal muscle lysate
Lane 2 : Human heart lysate
Lane 3 : HUVEC cell lysate
Lane 4 : C6 cell lysate
Lane 5 : RAW 264.7 cell lysate
Lane 6 : PC12 cell lysate
Lane 7 : NIH 3T3 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-rabbit HRP at 1/2000 dilution

Predicted band size: 33 kDa
Anti-Tropomyosin 1 (alpha) antibody [EPR5159] (ab133292)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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