Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Tuesday March 09, 2021

Anti-TPM4 antibody [EPR13316] (ab181085)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR13316] to TPM4
Suitable for: WB, IHC-P, IP
Knockout validated
Reacts with: Mouse, Human

Product name

Anti-TPM4 antibody [EPR13316]
See all TPM4 primary antibodies
Description

Rabbit monoclonal [EPR13316] to TPM4
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Mouse
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HEK293T, HeLa, A549, C2C12 whole cell lysate. IHC-P: Human cardiac muscle.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR13316
Isotype

IgG
Research areas

Western blot - Anti-TPM4 antibody [EPR13316] (ab181085)

All lanes : Anti-TPM4 antibody [EPR13316] (ab181085) at 1/1000 dilution

Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : TPM4 knockout HEK293T cell lysate
Lane 3 : HeLa cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 28 kDa
Observed band size: 29 kDa
why is the actual band size different from the predicted?

Lanes 1-3: Merged signal (red and green). Green - ab181085 observed at 29 kDa. Red - loading control ab8245 observed at 36 kDa.

ab181085 Anti-TPM4 antibody [EPR13316] was shown to specifically react with TPM4 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266606 (knockout cell lysate ab257758) was used. Wild-type and TPM4 knockout samples were subjected to SDS-PAGE. ab181085 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPM4 antibody [EPR13316] (ab181085)

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling TPM4 with ab181085 at a 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-TPM4 antibody [EPR13316] (ab181085)

All lanes : Anti-TPM4 antibody [EPR13316] (ab181085) at 1/5000 dilution

Lane 1 : HeLa whole cell lysate
Lane 2 : A549 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 28 kDa
Western blot - Anti-TPM4 antibody [EPR13316] (ab181085)

Anti-TPM4 antibody [EPR13316] (ab181085) at 1/20000 dilution + C2C12 whole cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 28 kDa
Immunoprecipitation - Anti-TPM4 antibody [EPR13316] (ab181085)

Western blot analysis on immunoprecipitation pellet from C2C12 cell lysate labeling TPM4 with ab181085. Right hand lane is the negative control.
Anti-TPM4 antibody [EPR13316] (ab181085)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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