Anti-TPM4 antibody - N-terminal (ab230880)
Key features and details
- Rabbit polyclonal to TPM4 - N-terminal
- Suitable for: WB, IHC-P, Flow Cyt (Intra)
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TPM4 antibody - N-terminal
See all TPM4 primary antibodies -
Description
Rabbit polyclonal to TPM4 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human
Predicted to work with: Pig
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Immunogen
Synthetic peptide within Human TPM4 aa 26-54 (N terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: P67936 -
Positive control
- IHC-P: Human placenta and skeletal muscle tissues. WB: U-251 MG, A549 and Jurkat cell lysates. Flow Cyt (Intra): U-251 MG cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TPM4 antibody - N-terminal (ab230880)All lanes : Anti-TPM4 antibody - N-terminal (ab230880) at 1/1000 dilution
Lane 1 : U-251 MG (Human brain glioma cell line) cell lysate
Lane 2 : A549 (Human lung carcinoma cell line) cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 28 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPM4 antibody - N-terminal (ab230880)Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for TPM4 using ab230880 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPM4 antibody - N-terminal (ab230880)Formalin-fixed, paraffin-embedded human placenta tissue stained for TPM4 using ab230880 at 1/100 dilution in immunohistochemical analysis.
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Flow Cytometry - Anti-TPM4 antibody - N-terminal (ab230880)Flow cytometric analysis of U-251 MG (Human brain glioma cell line) cells labeling TPM4 with ab230880 at 1/10 dilution(right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
