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Astana Biomed Group, an authorized Abcam distributor in Central Asia

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Tropomyosin

Anti-Tropomyosin 2 antibody [EPR12846] - BSA and Azide free (ab250178)

Anti-Tropomyosin 2 antibody [EPR12846] - BSA and Azide free (ab250178)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12846] to Tropomyosin 2 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human

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Overview

  • Product name

    Anti-Tropomyosin 2 antibody [EPR12846] - BSA and Azide free
    See all Tropomyosin 2 primary antibodies
  • Description

    Rabbit monoclonal [EPR12846] to Tropomyosin 2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HT-1080, fetal muscle and heart lysates, Human fetal muscle lysate. MYC and DDK-tagged Recombinant Human TPM2 protein (Full length)
  • General notes

    ab250178 is the carrier-free version of ab180176.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR12846
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Tropomyosin

Images

  • Immunoprecipitation - Anti-Tropomyosin 2 antibody [EPR12846] - BSA and Azide free (ab250178)
    Immunoprecipitation - Anti-Tropomyosin 2 antibody [EPR12846] - BSA and Azide free (ab250178)

    This data was developed using ab180176, the same antibody clone in a different buffer formulation.

    Western blot analysis on immunoprecipitation pellet from Human fetal muscle lysate labeling Tropomyosin 2 using ab180176 at 1/10.

  • Western blot - Anti-Tropomyosin 2 antibody [EPR12846] - BSA and Azide free (ab250178)
    Western blot - Anti-Tropomyosin 2 antibody [EPR12846] - BSA and Azide free (ab250178)
    All lanes : Anti-Tropomyosin 2 antibody [EPR12846] - C-terminal (ab180176) at 1/1000 dilution

    Lane 1 : Fetal heart lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HT-1080 cell lysate
    Lane 4 : Fetal muscle lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Standard HRP labeled goat anti-rabbit at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 33 kDa



    This data was developed using ab180176, the same antibody clone in a different buffer formulation.

     

  • Anti-Tropomyosin 2 antibody [EPR12846] - BSA and Azide free (ab250178)
    Anti-Tropomyosin 2 antibody [EPR12846] - BSA and Azide free (ab250178)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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