Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR357(2)] to delta 1 Catenin/CAS
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-delta 1 Catenin/CAS antibody [EPR357(2)]
    See all delta 1 Catenin/CAS primary antibodies

  • Description

    Rabbit monoclonal [EPR357(2)] to delta 1 Catenin/CAS

  • Host species

    Rabbit

  • Specificity

    The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human delta 1 Catenin/CAS aa 950 to the C-terminus. The exact sequence is proprietary.

  • Positive control

    • WB: HeLa, HEK-293T, RAW264.7, C2C12 and A431 cell lysates. IHC-P: Human breast carcinoma, ovarian cancer and colonic adenocarcinoma tissues. ICC/IF: A431 cells. Flow Cytometry: A431 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)
    Western blot - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)
    All lanes : Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514) at 1/1000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysates
    Lane 3 : RAW264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates
    Lane 4 : C2C12 (Mouse myoblasts myoblast) whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 108 kDa
    Observed band size: 70-120 kDa
    why is the actual band size different from the predicted?



    The multiple bands are isoforms caused by alternatively splicing  as was described in PMID 9653641 and are consistent with what has been descirbed in PMID 15077190.

  • Immunocytochemistry/ Immunofluorescence - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)
    Immunocytochemistry/ Immunofluorescence - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)

    Immunocytochemistry/ Immunofluorescence analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labeling delta 1 Catenin/CAS with purified ab92514 at 1:50 dilution (2.2 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian cancer tissue sections labeling delta 1 Catenin/CAS with purified ab92514 at 1:50 dilution (1.84 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunocytochemistry/ Immunofluorescence - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)
    Immunocytochemistry/ Immunofluorescence - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)

    A431 (Human epidermoid carcinoma cell line) cells stained for delta 1 Catenin/CAS using unpurified ab92514 at a dilution of 1/100 in ICC/IF.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)

    Paraffin embedded human breast carcinoma tissue (panel 1) and human colonic adenocarcinoma tissue (panel 2) labeled with unpurified ab92514 at 1/100 dilution.

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)
    Flow Cytometry - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)

    Flow Cytometry analysis of A431 (human epidermoid carcinoma) cells labeling delta 1 Catenin/CAS with unpurified ab92514 at 1/20 dilution (10 µg/ml) (Red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

  • OI-RD Scanning - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)
    OI-RD Scanning - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)
    Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)

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