Anti-delta 1 Catenin/CAS (phospho T310) antibody [EPR2382] (ab81318)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2382] to delta 1 Catenin/CAS (phospho T310)
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-delta 1 Catenin/CAS (phospho T310) antibody [EPR2382]
See all delta 1 Catenin/CAS primary antibodies -
Description
Rabbit monoclonal [EPR2382] to delta 1 Catenin/CAS (phospho T310) -
Host species
Rabbit -
Specificity
The antibody only detects delta 1 Catenin/CAS when phosphorylated on Threonine 310.
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Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human -
Immunogen
Synthetic peptide within Human delta 1 Catenin/CAS (phospho T310). The exact sequence is proprietary.
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Positive control
- 293T cell lysates treated with calyculin A Human breast carcinoma tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR2382 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-delta 1 Catenin/CAS (phospho T310) antibody [EPR2382] (ab81318) at 1/5000 dilution
Lane 1 : 293T cell lysates untreated
Lane 2 : 293T cell lysates treated with calyculin A
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti rabbit at 1/2000 dilution
Predicted band size: 108 kDa
Observed band size: 108 kDa
Additional bands at: 120 kDa. We are unsure as to the identity of these extra bands.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ab81318 at 1/100 dilution.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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