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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Catenins

Anti-delta 1 Catenin/CAS (phospho T310) antibody [EPR2382] (ab81318)

Price and availability

241 228 ₸

Availability

Order now and get it on Tuesday March 09, 2021

Anti-delta 1 Catenin/CAS (phospho T310) antibody [EPR2382] (ab81318)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2382] to delta 1 Catenin/CAS (phospho T310)
  • Suitable for: WB, IHC-P
  • Reacts with: Human

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Overview

  • Product name

    Anti-delta 1 Catenin/CAS (phospho T310) antibody [EPR2382]
    See all delta 1 Catenin/CAS primary antibodies
  • Description

    Rabbit monoclonal [EPR2382] to delta 1 Catenin/CAS (phospho T310)
  • Host species

    Rabbit
  • Specificity

    The antibody only detects delta 1 Catenin/CAS when phosphorylated on Threonine 310.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human delta 1 Catenin/CAS (phospho T310). The exact sequence is proprietary.

  • Positive control

    • 293T cell lysates treated with calyculin A Human breast carcinoma tissue
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR2382
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Catenins
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • Cytoplasmic

Images

  • Western blot - Anti-delta 1 Catenin/CAS (phospho T310) antibody [EPR2382] (ab81318)
    Western blot - Anti-delta 1 Catenin/CAS (phospho T310) antibody [EPR2382] (ab81318)
    All lanes : Anti-delta 1 Catenin/CAS (phospho T310) antibody [EPR2382] (ab81318) at 1/5000 dilution

    Lane 1 : 293T cell lysates untreated
    Lane 2 : 293T cell lysates treated with calyculin A

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti rabbit at 1/2000 dilution

    Predicted band size: 108 kDa
    Observed band size: 108 kDa
    Additional bands at: 120 kDa. We are unsure as to the identity of these extra bands.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-delta 1 Catenin/CAS (phospho T310) antibody [EPR2382] (ab81318)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-delta 1 Catenin/CAS (phospho T310) antibody [EPR2382] (ab81318)

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ab81318 at 1/100 dilution.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-delta 1 Catenin/CAS (phospho T310) antibody [EPR2382] (ab81318)
    Anti-delta 1 Catenin/CAS (phospho T310) antibody [EPR2382] (ab81318)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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