Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸ 10 µl 117 264 ₸

Order now and get it on Tuesday March 09, 2021

Anti-LYRIC/AEG1 antibody [EP4445] (ab124789)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP4445] to LYRIC/AEG1
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Mouse, Rat, Human

Product name

Anti-LYRIC/AEG1 antibody [EP4445]
See all LYRIC/AEG1 primary antibodies
Description

Rabbit monoclonal [EP4445] to LYRIC/AEG1
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human LYRIC/AEG1 aa 250-350. The exact sequence is proprietary.
Positive control
- HeLa, Jurkat, PC3, PC12 and NIH 3T3 cell lysates; Human brain and breast tissues; HeLa cells.
General notes
This product was previously labelled as LYRIC

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EP4445
Isotype

IgG
Research areas

Western blot - Anti-LYRIC/AEG1 antibody [EP4445] (ab124789)

All lanes : Anti-LYRIC/AEG1 antibody [EP4445] (ab124789) at 1/10000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : PC3 cell lysate
Lane 4 : PC12 cell lysate
Lane 5 : NIH 3T3 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 64 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYRIC/AEG1 antibody [EP4445] (ab124789)

ab124789 at 1/100 dilution staining LYRIC/AEG1 in paraffin-embedded Human breast tissues by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-LYRIC/AEG1 antibody [EP4445] (ab124789)

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling LYRIC/AEG1 with purified ab124789 at a dilution of 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei counterstained with DAPI (blue).

Control: PBS only.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYRIC/AEG1 antibody [EP4445] (ab124789)

ab124789 at 1/100 dilution staining LYRIC/AEG1 in paraffin-embedded Human brain tissues by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Anti-LYRIC/AEG1 antibody [EP4445] (ab124789)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com