Anti-LYRIC/AEG1 antibody (ab76742)
Key features and details
- Rabbit polyclonal to LYRIC/AEG1
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-LYRIC/AEG1 antibody
See all LYRIC/AEG1 primary antibodies -
Description
Rabbit polyclonal to LYRIC/AEG1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human LYRIC/AEG1 (N terminal).
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General notes
This product was previously labelled as LYRIC
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: Whole serum -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-LYRIC/AEG1 antibody (ab76742)All lanes : Anti-LYRIC/AEG1 antibody (ab76742) at 1/500 dilution
Lane 1 : Human heart tissue lysate - total protein (ab29431)
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg/ml per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 65 kDa
Observed band size: 65 kDa
Additional bands at: 32 kDa, 63 kDa, 67 kDa (possible post-translational modification). We are unsure as to the identity of these extra bands.
Exposure time: 3 minutesLYRIC/AEG1 contains a number of potential phosphorylation sites (SwissProt) which may explain the higher migrating band at 67 kDa.
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Immunocytochemistry/ Immunofluorescence - Anti-LYRIC/AEG1 antibody (ab76742)ICC/IF image of ab76742 stained Mcf7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76742, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
