All lanes : Anti-SORBS1 antibody (ab4551)

Lane 1 : Molecular weight marker (Bio-Rad Precision Plus Kaleidoscope standards)
Lane 2 : 10 µl Human plasma 1 (600-800 µg protein)
Lane 3 : 10 µl Human plasma 2 (600-800 µg protein)
Lane 4 : 10 µl Human White Blood cells 1 (30-50 µg protein)
Lane 5 : 10 µl Human White Blood cells 2 (30-50 µg protein)
Lane 6 : 10 µl HEK293 whole cell lysate (prepared by scrapping 10 cm confluent dish into 4ml of 2x sample buffer (Sigma)

Secondary
All lanes : Donkey anti-goat IgG (H+L) conjugated to Alexa-Fluor680 at 1/5000 dilution


ab4551 was tested in in human plasma, human white blood cells and HEK293 cells. Samples were run for 1h at 200 V using the Fisherbrand vertical complete gel unit, and transferred onto nitrocellulose 0.45um (1h, 100V, 4 C) using the Bio-rad Trans blot cell with plate electrodes. Membranes were blocked for 1 h at RT, then incubated overnight in primary antibody (1/200, 1/1000, 1/5000 or minus primary antibody) at 4 degrees C, with shaking. Following washing, membranes were incubated in secondary antibody (1/5000, Alexa-Fluor680 donkey anti-goat IgG, H+L) for 1 h at RT. Antibody binding was detected using LI-COR Bioscience's Odyssey Infrared Imaging system (intensity 5). The molecular weight of sorbs 1 was calculated using 'Compute pI/MW'from ExPASy tools based on the amino acid sequence according to NP_006425. The band indicated is approximately this predicted molecular weight, and is not present in the minus primary image, scanned at the same intensity.

Image courtesy of

ab4551 (2.5 µg/ml) staining SORBS1 in Human Skeletal Muscle by IHC-P.  Steamed antigen retrieval in citrate buffer pH6, AP-staining.  This image shows spotty staining on muscle fibres in longitudinal section.