Anti-SORBS1 antibody (ab224129)
Key features and details
- Rabbit polyclonal to SORBS1
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SORBS1 antibody
See all SORBS1 primary antibodies -
Description
Rabbit polyclonal to SORBS1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human SORBS1 aa 250-450.
Database link: Q9BX66 -
Positive control
- WB: Human tonsil tissue lysate. IHC-P: Human heart muscle, lymph node, cerebral cortex, colon and testis tissue. ICC/IF: U-2 OS cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-SORBS1 antibody (ab224129)
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for SORBS1 (green) using ab224129 at 4 µg/ml in ICC/IF.
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Western blot - Anti-SORBS1 antibody (ab224129)All lanes : Anti-SORBS1 antibody (ab224129) at 0.4 µg/ml
Lane 1 : Caco-2 cells lysates transfected with control siRNA
Lane 2 : Caco-2 cells lysates transfected with target specific siRNA probe #1
Lane 3 : Caco-2 cells lysates transfected with target specific siRNA probe #2
Predicted band size: 69,77,87,90,91,95,101,112,127,139,142,143 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SORBS1 antibody (ab224129)Paraffin-embedded human colon tissue stained for SORBS1 using ab224129 at 1/200 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SORBS1 antibody (ab224129)Paraffin-embedded human lymph node tissue stained for SORBS1 using ab224129 at 1/200 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SORBS1 antibody (ab224129)Paraffin-embedded human testis tissue stained for SORBS1 using ab224129 at 1/200 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SORBS1 antibody (ab224129)Paraffin-embedded human cerebral cortex tissue stained for SORBS1 using ab224129 at 1/200 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SORBS1 antibody (ab224129)Paraffin-embedded human heart muscle tissue stained for SORBS1 using ab224129 at 1/200 dilution in immunohistochemical analysis.
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Western blot - Anti-SORBS1 antibody (ab224129)Anti-SORBS1 antibody (ab224129) at 1/500 dilution + human tonsil tissue lysate
Developed using the ECL technique.
Predicted band size: 69,77,87,90,91,95,101,112,127,139,142,143 kDa
