Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-delta 1 Catenin/CAS antibody [YE372] - BSA and Azide free (ab247228)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [YE372] to delta 1 Catenin/CAS - BSA and Azide free
  • Suitable for: ICC, IHC-P, WB, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-delta 1 Catenin/CAS antibody [YE372] - BSA and Azide free
    See all delta 1 Catenin/CAS primary antibodies

  • Description

    Rabbit monoclonal [YE372] to delta 1 Catenin/CAS - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody is specific for human delta 1 Catenin/CAS (isoform 1ABC and 968AA). It also detects splice isoforms 1, 1A, 1B, 1C, 1AB, 1AC, 1BC, 2, 2A, 2B, 2C, 2AB, 2AC, 2BC and 2ABC of delta 1 Catenin/CAS.

  • Tested applications

    Suitable for: ICC, IHC-P, WB, Flow Cyt, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Epitope

    ab247228 reacts with an epitope located in the N terminal region of delta 1 Catenin/CAS

  • Positive control

    • Flow Cyt: HeLa cells IP: HeLa whole cell lysate.

  • General notes

    Ab247228 is the carrier-free version of ab32095. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247228 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-delta 1 Catenin/CAS antibody [YE372] - BSA and Azide free (ab247228)
    Western blot - Anti-delta 1 Catenin/CAS antibody [YE372] - BSA and Azide free (ab247228)
    Anti-delta 1 Catenin/CAS antibody [YE372] (ab32095) at 1/5000 dilution + HeLa cell lysate

    Predicted band size: 108 kDa
    Observed band size: 120 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab32095, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-delta 1 Catenin/CAS antibody [YE372] - BSA and Azide free (ab247228)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-delta 1 Catenin/CAS antibody [YE372] - BSA and Azide free (ab247228)
    This data was developed using ab32095, the same antibody clone in a different buffer formulation.ab32095 at a 1/100 dilution staining delta 1 Catenin/CAS in human breast carcinoma, using Immunohistochemistry, Paraffin Embedded Tissue. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Flow Cytometry - Anti-delta 1 Catenin/CAS antibody [YE372] - BSA and Azide free (ab247228)
    Flow Cytometry - Anti-delta 1 Catenin/CAS antibody [YE372] - BSA and Azide free (ab247228)
    This data was developed using ab32095, the same antibody clone in a different buffer formulation.
    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling delta 1 Catenin/CAS with purified ab32095 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).
  • Immunoprecipitation - Anti-delta 1 Catenin/CAS antibody [YE372] - BSA and Azide free (ab247228)
    Immunoprecipitation - Anti-delta 1 Catenin/CAS antibody [YE372] - BSA and Azide free (ab247228)

    This data was developed using ab32095, the same antibody clone in a different buffer formulation.
    Purified ab32095 at 1/50 dilution (2µg) immunoprecipitating delta 1 Catenin/CAS in HeLa whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab32095 + HeLa whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32095 in HeLa whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 120 kDa
    Multi-bands could be isoforms of delta 1 Catenin based on Uniprot.

  • Anti-delta 1 Catenin/CAS antibody [YE372] - BSA and Azide free (ab247228)
    Anti-delta 1 Catenin/CAS antibody [YE372] - BSA and Azide free (ab247228)

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