Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-gamma Catenin antibody [11E4] - BSA and Azide free (ab269564)

Key features and details

  • Mouse monoclonal [11E4] to gamma Catenin - BSA and Azide free
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-gamma Catenin antibody [11E4] - BSA and Azide free
    See all gamma Catenin primary antibodies

  • Description

    Mouse monoclonal [11E4] to gamma Catenin - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: FFPE normal human cervix tissue sections. ICC-IF: A431 cell line. WB: A431 and HeLa cells

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab269564 is the carrier-free version of ab231304. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-gamma Catenin antibody [11E4] - BSA and Azide free (ab269564)
    Western blot - Anti-gamma Catenin antibody [11E4] - BSA and Azide free (ab269564)
    All lanes : Anti-gamma Catenin antibody [11E4] (ab231304) at 1 µg/ml

    Lane 1 : A431 whole cell lysate
    Lane 2 : Mouse liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 82 kDa



    This blot was produced using a 4-12% Bis-tris under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was blocked for an hour using 3% milk before ab231304 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at a 1ug/ml concentration and 1/20000 dilution respectively. Antibody binding was detected using Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    This image was produced using the same antibody clone but in a different formulation ab231304, PBS and sodium azide.

  • Immunocytochemistry/ Immunofluorescence - Anti-gamma Catenin antibody [11E4] - BSA and Azide free (ab269564)
    Immunocytochemistry/ Immunofluorescence - Anti-gamma Catenin antibody [11E4] - BSA and Azide free (ab269564)

    ab231304 staining gamma Catenin in A431 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1%PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab231304 at 1μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150084, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This image was produced using the same antibody clone but in a different formulation ab231304, PBS and sodium azide.

  • Western blot - Anti-gamma Catenin antibody [11E4] - BSA and Azide free (ab269564)
    Western blot - Anti-gamma Catenin antibody [11E4] - BSA and Azide free (ab269564)
    All lanes :

    Lane 1 : A431 whole cell lysate
    Lane 2 : HeLa whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 82 kDa



    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before ab231304 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at a 1ug/ml concentration and 1/10000 dilution respectively. Antibody binding was detected using Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [11E4] - BSA and Azide free (ab269564)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [11E4] - BSA and Azide free (ab269564)

    IHC image of gamma Catenin staining in a section of formalin-fixed paraffin-embedded normal human cervix* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab231304, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    This image was produced using the same antibody clone but in a different formulation ab231304, PBS and sodium azide.

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