Anti-gamma Catenin antibody [EPR17310-48] - BSA and Azide free (ab251317)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17310-48] to gamma Catenin - BSA and Azide free
- Suitable for: WB, ICC, IHC-P, Flow Cyt (Intra)
- Reacts with: Human
Overview
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Product name
Anti-gamma Catenin antibody [EPR17310-48] - BSA and Azide free
See all gamma Catenin primary antibodies -
Description
Rabbit monoclonal [EPR17310-48] to gamma Catenin - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC, IHC-P, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC: HeLa cells
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General notes
ab251317 is the carrier-free version of ab200661.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17310-48 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-gamma Catenin antibody [EPR17310-48] (ab200661) at 1/20000 dilution
Lane 1 : Human fetal heart lysates
Lane 2 : Human fetal kidney lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 82 kDa
Observed band size: 82 kDa
Exposure time: 3 minutesThis data was developed using ab200661, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [EPR17310-48] - BSA and Azide free (ab251317)
This data was developed using ab200661, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling gamma Catenin with ab200661 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on Human kidney tissue is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
Anti-gamma Catenin antibody [EPR17310-48] (ab200661) at 1/5000 dilution + Human fetal skin lysates at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 82 kDa
Observed band size: 82 kDa
Exposure time: 5 secondsThis data was developed using ab200661, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [EPR17310-48] - BSA and Azide free (ab251317)This data was developed using ab200661, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling gamma Catenin with ab200661 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on Human cervix carcinoma tissue is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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All lanes : Anti-gamma Catenin antibody [EPR17310-48] (ab200661) at 1/5000 dilution
Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 2 : T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 82 kDa
Observed band size: 82 kDa
Exposure time: 5 secondsThis data was developed using ab200661, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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This data was developed using the same antibody clone in a different buffer formulation (ab200661).
Immunocytochemistry analysis of Hela (Human cervix adenocarcinoma epithelial cell) labeling gamma Catenin with purified ab200661 at 1/400 dilution. Cells were fixed with 100% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. was used as counterstain. Nuclei were stained blue with DAPI.
Negative control: PBS instead of the primary antibody. -
This data was developed using ab200661, the same antibody clone in a different buffer formulation.Flow cytometry analysis of HeLa cells labelling gamma Catenin (red) with purified ab200661at dilution of 1/40. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.
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