Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-gamma Catenin antibody [EPR17310-48] - BSA and Azide free (ab251317)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17310-48] to gamma Catenin - BSA and Azide free
  • Suitable for: WB, ICC, IHC-P, Flow Cyt (Intra)
  • Reacts with: Human

Overview

  • Product name

    Anti-gamma Catenin antibody [EPR17310-48] - BSA and Azide free
    See all gamma Catenin primary antibodies

  • Description

    Rabbit monoclonal [EPR17310-48] to gamma Catenin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC, IHC-P, Flow Cyt (Intra)more details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC: HeLa cells

  • General notes

    ab251317 is the carrier-free version of ab200661.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-gamma Catenin antibody [EPR17310-48] - BSA and Azide free (ab251317)
    Western blot - Anti-gamma Catenin antibody [EPR17310-48] - BSA and Azide free (ab251317)
    All lanes : Anti-gamma Catenin antibody [EPR17310-48] (ab200661) at 1/20000 dilution

    Lane 1 : Human fetal heart lysates
    Lane 2 : Human fetal kidney lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 82 kDa
    Observed band size: 82 kDa


    Exposure time: 3 minutes


    This data was developed using ab200661, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [EPR17310-48] - BSA and Azide free (ab251317)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [EPR17310-48] - BSA and Azide free (ab251317)

    This data was developed using ab200661, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling gamma Catenin with ab200661 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on Human kidney tissue is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Western blot - Anti-gamma Catenin antibody [EPR17310-48] - BSA and Azide free (ab251317)
    Western blot - Anti-gamma Catenin antibody [EPR17310-48] - BSA and Azide free (ab251317)
    Anti-gamma Catenin antibody [EPR17310-48] (ab200661) at 1/5000 dilution + Human fetal skin lysates at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 82 kDa
    Observed band size: 82 kDa


    Exposure time: 5 seconds


    This data was developed using ab200661, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [EPR17310-48] - BSA and Azide free (ab251317)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [EPR17310-48] - BSA and Azide free (ab251317)
    This data was developed using ab200661, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling gamma Catenin with ab200661 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on Human cervix carcinoma tissue is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Western blot - Anti-gamma Catenin antibody [EPR17310-48] - BSA and Azide free (ab251317)
    Western blot - Anti-gamma Catenin antibody [EPR17310-48] - BSA and Azide free (ab251317)
    All lanes : Anti-gamma Catenin antibody [EPR17310-48] (ab200661) at 1/5000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 2 : T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 82 kDa
    Observed band size: 82 kDa


    Exposure time: 5 seconds


    This data was developed using ab200661, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry - Anti-gamma Catenin antibody [EPR17310-48] - BSA and Azide free (ab251317)
    Immunocytochemistry - Anti-gamma Catenin antibody [EPR17310-48] - BSA and Azide free (ab251317)

    This data was developed using the same antibody clone in a different buffer formulation (ab200661).
    Immunocytochemistry analysis of Hela (Human cervix adenocarcinoma epithelial cell) labeling gamma Catenin with purified ab200661 at 1/400 dilution. Cells were fixed with 100% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. was used as counterstain. Nuclei were stained blue with DAPI.
    Negative control: PBS instead of the primary antibody.

  • Flow Cytometry - Anti-gamma Catenin antibody [EPR17310-48] - BSA and Azide free (ab251317)
    Flow Cytometry - Anti-gamma Catenin antibody [EPR17310-48] - BSA and Azide free (ab251317)
    This data was developed using ab200661, the same antibody clone in a different buffer formulation.Flow cytometry analysis of HeLa cells labelling gamma Catenin (red) with purified ab200661at dilution of 1/40. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.
  • Anti-gamma Catenin antibody [EPR17310-48] - BSA and Azide free (ab251317)
    Anti-gamma Catenin antibody [EPR17310-48] - BSA and Azide free (ab251317)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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