Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] - BSA and Azide free (ab238957)

Key features and details

  • Rabbit monoclonal [EP2122Y] to Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) - BSA and Azide free
  • Suitable for: WB, IHC-P, Dot blot
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] - BSA and Azide free
    See all Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) primary antibodies

  • Description

    Rabbit monoclonal [EP2122Y] to Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    ab238957 will detect Ezrin, Radixin and Moesin. This antibody detects the phosphorylated target and not the non-phosphorylated epitope.

  • Tested applications

    Suitable for: WB, IHC-P, Dot blotmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC: human breast adenocarcinoma.

  • General notes

    Ab238957 is the carrier-free version of ab76247. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab238957 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Dot Blot - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] - BSA and Azide free (ab238957)
    Dot Blot - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] - BSA and Azide free (ab238957)

    Dot blot analysis of Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) phospho peptide (Lane 1), Non-phospho peptide (Lane 2), labelling Ezrin (phospho Thr567)/ Radixin (phospho Thr564)/ Moesin (phospho Thr558) with ab76247 at a dilution of 1/1000. Peroxidase conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.

    Blocking and diluting buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    The sequence of non-phospho peptide: CGRDKYKTLRQIR.
    The sequence of phospho peptide: CGRDKYK-pT-LRQIR.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76247).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] - BSA and Azide free (ab238957)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] - BSA and Azide free (ab238957)

    Immunohistochemical analysis of paraffin-embedded human breast adenocarcinoma with ab76247 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76247).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] - BSA and Azide free (ab238957)

Clear all

Recently viewed products

© 2021 Astana Biomed Group LLP. All rights reserved.