Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] - BSA and Azide free (ab238957)
![Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] - BSA and Azide free (ab238957)](https://www.abcam.com/ps/products/238/ab238957/Images/ab238957-328502-anti-ezrin-pthr567-radixin-pthr564-moesin-pthr558-antibody-ep2122y-dot-blot.jpg "Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] - BSA and Azide free (ab238957)")
Key features and details
- Rabbit monoclonal [EP2122Y] to Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) - BSA and Azide free
- Suitable for: WB, IHC-P, Dot blot
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] - BSA and Azide free
See all Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) primary antibodies -
Description
Rabbit monoclonal [EP2122Y] to Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) - BSA and Azide free -
Host species
Rabbit -
Specificity
ab238957 will detect Ezrin, Radixin and Moesin. This antibody detects the phosphorylated target and not the non-phosphorylated epitope. -
Tested applications
Suitable for: WB, IHC-P, Dot blotmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC: human breast adenocarcinoma.
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General notes
Ab238957 is the carrier-free version of ab76247. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab238957 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP2122Y -
Isotype
IgG -
Research areas
Images
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Dot Blot - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] - BSA and Azide free (ab238957)
Dot blot analysis of Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) phospho peptide (Lane 1), Non-phospho peptide (Lane 2), labelling Ezrin (phospho Thr567)/ Radixin (phospho Thr564)/ Moesin (phospho Thr558) with ab76247 at a dilution of 1/1000. Peroxidase conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.
Blocking and diluting buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
The sequence of non-phospho peptide: CGRDKYKTLRQIR.
The sequence of phospho peptide: CGRDKYK-pT-LRQIR.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76247).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] - BSA and Azide free (ab238957)](https://www.abcam.com/ps/products/238/ab238957/Images/ab238957-317735-anti-ezrin-pthr567-radixin-pthr564-moesin-pthr558-antibody-ep2122y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] - BSA and Azide free (ab238957)Immunohistochemical analysis of paraffin-embedded human breast adenocarcinoma with ab76247 at 1/100 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76247).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
